Human glutathione S-transferase mu 4 ELISA Kit | GSTM4 elisa kit

Human Glutathione S-transferase Mu 4, GSTM4 ELISA Kit

Cat. Num. AAA9333779

• Gene Names: GSTM4; GTM4; GSTM4-4
• Reactivity: Human
• Synonyms: glutathione S-transferase mu 4; Human Glutathione S-transferase Mu 4; GSTM4 ELISA Kit; Human Glutathione S-transferase Mu 4 (GSTM4) ELISA kit; GSTM4-4; GTM4; MGC131945; MGC9247; GST class-mu 4; GTS-Mu2; S- (hydroxyalkyl) glutathione lyase M4; glutathione S-alkyltransferase M4; glutathione S-aralkyltransferase M4; glutathione S-aryltransferase M4; ; GSTM4 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human GSTM4 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 0.625 ng/ml - 20 ng/ml.
• Sensitivity: 0.1 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated GSTM4 concentrations in Human serum, plasma and other body fluids. Using Purified Human GSTM4 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GSTM4 and GSTM4 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GSTM4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for GSTM4 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated GSTM4 concentrations in Human serum, plasma and other body fluids. Using Purified Human GSTM4 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GSTM4 and GSTM4 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GSTM4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4504179
• NCBI GeneID: 2948
• NCBI Accession #: NP_000841.1
• NCBI GenBank Nucleotide #: NM_000850.4
• UniProt Accession #: Q03013; Q05465; Q32NC1; Q4JNT8; Q6FH87; A8K765
• Molecular Weight: 25,561 Da
• NCBI Official Full Name: glutathione S-transferase Mu 4 isoform 1
• NCBI Official Synonym Full Names: glutathione S-transferase mu 4
• NCBI Official Symbol: GSTM4
• NCBI Official Synonym Symbols: GTM4; GSTM4-4
• NCBI Protein Information: glutathione S-transferase Mu 4; GST-Mu2; GTS-Mu2; GST class-mu 4; glutathione S-transferase M4; glutathione S-aryltransferase M4; glutathione S-alkyltransferase M4; glutathione S-aralkyltransferase M4; S-(hydroxyalkyl)glutathione lyase M4
• UniProt Protein Name: Glutathione S-transferase Mu 4
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: GSTM4
• UniProt Entry Name: GSTM4_HUMAN

NCBI Description

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Diversification of these genes has occurred in regions encoding substrate-binding domains, as well as in tissue expression patterns, to accommodate an increasing number of foreign compounds. Multiple transcript variants, each encoding a distinct protein isoform, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

GSTM4: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Active on 1- chloro-2,4-dinitrobenzene. Belongs to the GST superfamily. Mu family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Xenobiotic Metabolism - drug metabolism - cytochrome P450; Other Amino Acids Metabolism - glutathione; Transferase; EC 2.5.1.18; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: cytoplasm; cytosol

Molecular Function: protein homodimerization activity; enzyme binding; glutathione transferase activity; glutathione binding

Biological Process: glutathione metabolic process; nitrobenzene metabolic process; xenobiotic metabolic process; xenobiotic catabolic process