GSTM1 recombinant protein

GSTM1, 1-181aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA205688

• Gene Names: GSTM1; MU; H-B; GST1; GTH4; GTM1; MU-1; GSTM1-1; GSTM1a-1a; GSTM1b-1b
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: GSTM1; 1-181aa; Human; His tag; E Coli (Denatured); Glutathione S-transferase mu 1; GST1; GSTM1-1; GSTM1a-1a; GSTM1b-1b; GTH4; GTM1; H-B; Mu; Mu-1; GSTM1 recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH RSMPMILGYW DIRGLAHAIR LLLEYTDSSY EEKKYTMGDA PDYDRSQWLN EKFKLGLDFP NLPYLIDGAH KITQSNAILC YIARKHNLCG ETEEEKIRVD ILENQTMDNH MQLGMICYNP EFEKLKPKYL EELPEKLKLY SEFLGKRPWF AGNKGLEKIS AYMKSSRFLP RPVFSKMAVW GNK
• Sequence Length: 181

• Applicable Applications for GSTM1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for GSTM1 recombinant protein

Glutathione S-transferase mu 1 isoform 2, also known as GSTM1, is a glutathione S-transferase that belongs to the mu class. GSTs (glutathione S-transferase) are differentially expressed in lung, liver and kidney tissue and, notably, three isoforms (GSTA1-1, GSTA1-4 and GSTM1) localize to the mitochondria in addition to the cytoplasm. The GSTM1 of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Recombinant human GSTM1 protein, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for GSTM1 recombinant protein

Metabolism

References

Bartley P A., et al. (2003) Oncogene. 22:7570-7575.; Moretti M., et al. (2007) BMC Public Health. 7:270.;

NCBI and Uniprot Product Information

• NCBI GI #: 23065547
• NCBI GeneID: 2944
• NCBI Accession #: NP_666533
• NCBI GenBank Nucleotide #: NM_146421.2
• UniProt Accession #: P09488; Q5GHG0; Q6FH88; Q8TC98; Q9UC96
• Molecular Weight: 23.6 kDa (203aa)
• NCBI Official Full Name: glutathione S-transferase Mu 1 isoform 2
• NCBI Official Synonym Full Names: glutathione S-transferase mu 1
• NCBI Official Symbol: GSTM1
• NCBI Official Synonym Symbols: MU; H-B; GST1; GTH4; GTM1; MU-1; GSTM1-1; GSTM1a-1a; GSTM1b-1b
• NCBI Protein Information: glutathione S-transferase Mu 1
• UniProt Protein Name: Glutathione S-transferase Mu 1
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: GSTM1
• UniProt Synonym Gene Names: GST1

NCBI Description

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Null mutations of this class mu gene have been linked with an increase in a number of cancers, likely due to an increased susceptibility to environmental toxins and carcinogens. Multiple protein isoforms are encoded by transcript variants of this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.