GST A3-3 recombinant protein

GST A3-3, Recombinant Human

Cat. Num. AAA483025

• Gene Names: GSTA3; GTA3; GSTA3-3
• Purity: 95% as assessed by inspection on a Coomassie Blue-stained SDS-PAGE gel.
• Synonyms: GST A3-3; Recombinant Human; Recombinant Human GST A3-3; GST A3-3 recombinant protein

• Host: Human recombinant protein expressed in E Coli.
• Specificity: Specific Activity: 61 U/mg using spectrophotometric determination of 1-chloro- 2,4-dinitrobenzene (CDNB) conjugation with reduced glutathione (1 mM) in 100 mM NaPO4 (pH 6.5) at room temperature.
• Purity/Purification: 95% as assessed by inspection on a Coomassie Blue-stained SDS-PAGE gel.
• Concentration: 1.0 mg/mL total protein using the Bradford protein assay with BSA as a standard. (varies by lot)
• Sequence Length: 222

• Storage Buffer: 1.0 mg/mL total protein using the Bradford protein assay with BSA as a standard.
• Procedural Notes: This purified product exhibits high enzymatic activity for CDNB, the synthetic substrate that is most commonly used for GST analyses. However, as is widely known for GST activity analysis using CDNB as a substrate, low concentrations (high dilutions) of the enzyme result in lower activity values. In contrast, initial velocities are much higher for more concentrated levels of enzyme but the rate decreases rapidly. Therefore, activity toward CDNB and these considerations provide guidance when assaying this product under low concentrations or when using substrates other than CDNB.
• Preparation and Storage: At -20 degree C; AVOID MULTIPLE FREEZE-THAW CYCLES.

NCBI and Uniprot Product Information

• NCBI GI #: 21264437
• NCBI GeneID: 2940
• UniProt Accession #: Q068V6; O43468; Q8WWA8; Q9H415; Q16772
• Molecular Weight: ~25 kDa
• NCBI Official Full Name: Glutathione S-transferase A3
• NCBI Official Synonym Full Names: glutathione S-transferase alpha 3
• NCBI Official Symbol: GSTA3
• NCBI Official Synonym Symbols: GTA3; GSTA3-3
• NCBI Protein Information: glutathione S-transferase A3; GST class-alpha member 3; S-(hydroxyalkyl)glutathione lyase A3; glutathione S-alkyltransferase A3; glutathione S-aralkyltransferase A3; glutathione S-aryltransferase A3; glutathione S-transferase A3-3
• UniProt Protein Name: Glutathione S-transferase A3
• UniProt Gene Name: GSTA3
• UniProt Entry Name: GSTA3_HUMAN

NCBI Description

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class genes that are located in a cluster mapped to chromosome 6. Genes of the alpha class are highly related and encode enzymes with glutathione peroxidase activity. However, during evolution, this alpha class gene diverged accumulating mutations in the active site that resulted in differences in substrate specificity and catalytic activity. The enzyme encoded by this gene catalyzes the double bond isomerization of precursors for progesterone and testosterone during the biosynthesis of steroid hormones. An additional transcript variant has been identified, but its full length sequence has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

GSTA3: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Catalyzes isomerization reactions that contribute to the biosynthesis of steroid hormones. Efficiently catalyze obligatory double-bond isomerizations of delta(5)-androstene-3,17-dione and delta(5)- pregnene-3,20-dione, precursors to testosterone and progesterone, respectively. Belongs to the GST superfamily. Alpha family.

Protein type: Xenobiotic Metabolism - metabolism by cytochrome P450; Other Amino Acids Metabolism - glutathione; Transferase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 2.5.1.18

Chromosomal Location of Human Ortholog: 6p12.1

Cellular Component: cytosol

Molecular Function: glutathione transferase activity

Biological Process: glutathione metabolic process; xenobiotic metabolic process