Rat GSTT2 ELISA Kit | GST T2 elisa kit
Rat GSTT2 (Glutathione S Transferase Theta 2) ELISA Kit
Reactivity
Rat
Synonyms
GSTT2; Rat GSTT2 (Glutathione S Transferase Theta 2) ELISA Kit; GST T2 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat GST T2. No significant cross-reactivity or interference between Rat GST T2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rat GST T2 and all the analogues, therefore, cross reaction may still exist.
Sequence Length
244
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
1.563-100mIU/mL
Sensitivity
Min: 0.938mIU/mL; Max: 100mIU/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GST T2 elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GST T2 in Rat serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GST T2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat GST T2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GST T2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat GST T2. You can calculate the concentration of Rat GST T2 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GST T2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat GST T2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GST T2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat GST T2. You can calculate the concentration of Rat GST T2 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,506 Da
NCBI Official Full Name
glutathione S-transferase theta-2
NCBI Official Synonym Full Names
glutathione S-transferase theta 2 (gene/pseudogene)
NCBI Official Symbol
GSTT2
NCBI Protein Information
glutathione S-transferase theta-2; GST class-theta-2
UniProt Protein Name
Glutathione S-transferase theta-2
UniProt Gene Name
GSTT2
UniProt Entry Name
GST2_HUMAN
NCBI Description
Glutathione S-transferase (GSTs) theta 2 (GSTT2) is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: Alpha, Mu, Pi, Theta, and Zeta. The theta class members GSTT1 and GSTT2 share 55% amino acid sequence identity and both are thought to have an important role in human carcinogenesis. The theta genes have a similar structure, being composed of five exons with identical exon/intron boundaries. [provided by RefSeq, Jul 2008]
Uniprot Description
GST2: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a sulfatase activity. Belongs to the GST superfamily. Theta family.
Protein type: EC 2.5.1.18
Chromosomal Location of Human Ortholog: 22q11.23
Cellular Component: cytoplasm
Molecular Function: glutathione transferase activity
Biological Process: metabolic process
