Gastrin Releasing Peptide Receptor Recombinant Protein | GRPR recombinant protein

Recombinant Gastrin Releasing Peptide Receptor (GRPR)

Cat. Num. AAA2033159

• Gene Names: GRPR; BB2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Gastrin Releasing Peptide Receptor; Recombinant Gastrin Releasing Peptide Receptor (GRPR); BB2; GRP-preferring bombesin receptor; GRPR recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: MAPNNCSHLN LDVDPFLSCN DTFNQSLSPP KMDNWFHPGF IYVIPAVYGL IIVIGLIGNITLIKIFCTVK SMRNVPNLFI SSLALGDLLL LVTCAPVDAS KYLADRWLFG RIGCKL
• Sequence Length: 384

• Applicable Applications for GRPR recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 42.8kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Met1~Leu116
• Organism Species: Mouse
• Tag: two N-terminal Tags, His-tag and GST-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4885361
• NCBI GeneID: 2925
• NCBI Accession #: NP_005305.1
• NCBI GenBank Nucleotide #: NM_005314.2
• Molecular Weight: 43,199 Da
• NCBI Official Full Name: gastrin-releasing peptide receptor
• NCBI Official Synonym Full Names: gastrin releasing peptide receptor
• NCBI Official Symbol: GRPR
• NCBI Official Synonym Symbols: BB2
• NCBI Protein Information: gastrin-releasing peptide receptor
• UniProt Protein Name: Gastrin-releasing peptide receptor
• Protein Family: Gastrin-releasing peptide receptor
• UniProt Gene Name: GRPR
• UniProt Synonym Gene Names: GRP-R
• UniProt Entry Name: GRPR_HUMAN

NCBI Description

Gastrin-releasing peptide (GRP) regulates numerous functions of the gastrointestinal and central nervous systems, including release of gastrointestinal hormones, smooth muscle cell contraction, and epithelial cell proliferation and is a potent mitogen for neoplastic tissues. The effects of GRP are mediated through the gastrin-releasing peptide receptor. This receptor is a glycosylated, 7-transmembrane G-protein coupled receptor that activates the phospholipase C signaling pathway. The receptor is aberrantly expressed in numerous cancers such as those of the lung, colon, and prostate. An individual with autism and multiple exostoses was found to have a balanced translocation between chromosome 8 and a chromosome X breakpoint located within the gastrin-releasing peptide receptor gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GRPR: Receptor for gastrin releasing peptide (GRP). This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: Xp22.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: peptide receptor activity, G-protein coupled

Biological Process: cell proliferation; G-protein coupled receptor protein signaling pathway; regulation of cell proliferation