GroES Recombinant Protein
GroES, Recombinant (Chaperonin 10, cpn10, Heat Shock Protein 10, HSP10)
Applications
Western Blot
Purity
PurifiedPurified by chromatography. > 75-80% as determined by SDS-PAGE analysis.
Synonyms
GroES; Recombinant (Chaperonin 10; cpn10; Heat Shock Protein 10; HSP10); GroES recombinant protein
Host
Recombinant (E Coli).
Purity/Purification
Purified
Purified by chromatography. > 75-80% as determined by SDS-PAGE analysis.
Purified by chromatography. > 75-80% as determined by SDS-PAGE analysis.
Form/Format
Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 100mM sodium chloride, 10mM magnesium chloride, 5mM DTT, 0.05% sodium azide.
Applicable Applications for GroES recombinant protein
Western Blot (WB)
Application Notes
Suitable for use in Functional Activity Assay and Western Blot.
Dilution: Western Blot: 50-100ng per lane.
Use G8980-25 GroES Pab Rbx
Dilution: Western Blot: 50-100ng per lane.
Use G8980-25 GroES Pab Rbx
Preparation and Storage
Aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
Related Product Information for GroES recombinant protein
The E.coli heat shock proteins GroEL and GroES are two of the most thoroughly characterized members of a class of proteins known collectively as "molecular chaperones". GroEL (cpn60; Hsp60 homolog) is an acidic 60kD E. coli heat shock protein which possesses a weak ATPase activity. In its native form, GroEL exists as a 14-mer consisting of two stacked rings each composed of seven identical subunits of Mr ~57,250. GroES (cpn10; Hsp10 homolog) consists of a single ring of seven identical subunits of Mr ~10,350 (2,3). Molecular chaperones, such as GroEL and GroES, are thought to function by interacting with transiently exposed interactive surfaces during their nascent synthesis, folding, transport and oligomerization. The net effect of chaperone participation in these processes is a reduction in inappropriate protein-protein interactions that might produce a nonfunctional structure. The GroEL and GroES genes, constituting the GroE operon, are members of the heat shock regulon of E. coli (7,8). Synthesis of the GroE protein, which accounts for approximately 1% of the cellular protein at 37 degree C, increases to about 10% of the total protein content upon shifting the growth temperature to 46 degree C.
Product Categories/Family for GroES recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
groes
Uniprot Description
Binds to Cpn60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.
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Product Notes
The GroES (Catalog #AAA638315) is a Recombinant Protein produced from Recombinant (E Coli). and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GroES can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Functional Activity Assay and Western Blot. Dilution: Western Blot: 50-100ng per lane. Use G8980-25 GroES Pab Rbx. Researchers should empirically determine the suitability of the GroES for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GroES, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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