Mouse G protein-coupled receptor kinase 5 ELISA Kit | GRK5 elisa kit

Mouse G protein-coupled receptor kinase 5, GRK5 ELISA Kit

Cat. Num. AAA9336679

• Gene Names: Grk5; Gprk5
• Reactivity: Mouse
• Synonyms: G protein-coupled receptor kinase 5; Mouse G protein-coupled receptor kinase 5; GRK5 ELISA Kit; Mouse G protein-coupled receptor kinase 5 (GRK5) ELISA kit; GPRK5; ; GRK5 elisa kit

• Reactivity: Mouse
• Specificity: No significant cross-reactivity or interference between Mouse GRK5 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 62.5 pg/ml - 2000 pg/ml.
• Sensitivity: 10 pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated GRK5 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse GRK5 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GRK5 and GRK5 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GRK5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for GRK5 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated GRK5 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse GRK5 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GRK5 and GRK5 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GRK5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 159032059
• NCBI GeneID: 14773
• NCBI Accession #: NP_061357.3
• NCBI GenBank Nucleotide #: NM_018869.3
• UniProt Accession #: Q8VEB1; O70292; O70297
• Molecular Weight: 67,732 Da
• NCBI Official Full Name: G protein-coupled receptor kinase 5
• NCBI Official Synonym Full Names: G protein-coupled receptor kinase 5
• NCBI Official Symbol: Grk5
• NCBI Official Synonym Symbols: Gprk5
• NCBI Protein Information: G protein-coupled receptor kinase 5; g protein-coupled receptor kinase GRK5
• UniProt Protein Name: G protein-coupled receptor kinase 5
• Protein Family: G protein-coupled receptor kinase
• UniProt Gene Name: Grk5
• UniProt Synonym Gene Names: Gprk5
• UniProt Entry Name: GRK5_MOUSE

Uniprot Description

GRK5: Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein- coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70- interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2- mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G- protein-coupled receptor, LRP6 during Wnt signaling (in vitro). Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.16; Kinase, protein; Protein kinase, AGC; AGC group; GRK family; GRK subfamily

Cellular Component: nucleoplasm; nuclear membrane; focal adhesion; membrane; cytoplasm; plasma membrane; nucleus

Molecular Function: transferase activity; protein serine/threonine kinase activity; G-protein coupled receptor kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; lipid binding; ATP binding; protein kinase activity

Biological Process: desensitization of G-protein coupled receptor protein signaling pathway; tachykinin signaling pathway; Wnt receptor signaling pathway; apoptosis; protein amino acid autophosphorylation; signal transduction; phosphorylation; protein amino acid phosphorylation; regulation of G-protein coupled receptor protein signaling pathway; negative regulation of apoptosis