G Protein Coupled Receptor Kinase 4 Recombinant Protein | GRK4 recombinant protein

Recombinant G Protein Coupled Receptor Kinase 4 (GRK4)

Cat. Num. AAA2029619

• Gene Names: Grk4; Gprk2l
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: G Protein Coupled Receptor Kinase 4; Recombinant G Protein Coupled Receptor Kinase 4 (GRK4); GRK4 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; FRHY RVLGKGGFGE VCACQVRATG KMYACKKLQK KRIKKRKGEA MALNEKRILE KVQSRFVVSL AYAYETKDAL CLVLTIMNGG DLKFHIYNLG NPGFDEQRAV FYAAELCCGL EDLQRERIVY RDLKPENILL DDRGHIRISD LGLATEIPEG QRVRGRVGTV GYMAPEVVNN EKYTFSPDWW GLGCLIYEMI QGHSPFKKYK EKVKWEEVDQ RIKNDTEEYS EKFSEDAKSICRMLLTKNPS KRLGCRGEGA AGVKQHPVF
• Sequence Length: 575

• Applicable Applications for GRK4 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 33.8kDa
• Accurate Molecular Mass (KD): 34kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag, T7 tag
• Organism Species: Homo sapiens (Human)
• Fragment: Met1~Cys230
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 12621084
• NCBI GeneID: 59077
• NCBI Accession #: NP_075217.1
• NCBI GenBank Nucleotide #: NM_022928.1
• Molecular Weight: 62,668 Da
• NCBI Official Full Name: G protein-coupled receptor kinase 4
• NCBI Official Synonym Full Names: G protein-coupled receptor kinase 4
• NCBI Official Symbol: Grk4
• NCBI Official Synonym Symbols: Gprk2l
• NCBI Protein Information: G protein-coupled receptor kinase 4
• UniProt Protein Name: G protein-coupled receptor kinase 4
• Protein Family: G protein-coupled receptor kinase
• UniProt Gene Name: Grk4
• UniProt Synonym Gene Names: Gprk2l
• UniProt Entry Name: GRK4_RAT

NCBI Description

kinase that phosphorylates agonist-occupied form of G protein-coupled receptors and may be involved in testicular and spermatocyte development [RGD, Feb 2006]

Uniprot Description

GRK4: Specifically phosphorylates the activated forms of G protein-coupled receptors. GRK4-alpha can phosphorylate rhodopsin and its activity is inhibited by calmodulin; the other three isoforms do not phosphorylate rhodopsin and do not interact with calmodulin. GRK4-alpha and GRK4-gamma phosphorylate DRD3. Phosphorylates ADRB2. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; EC 2.7.11.16; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; GRK family; GRK subfamily

Cellular Component: cell soma; dendrite; cell cortex; cytosol

Molecular Function: G-protein coupled receptor kinase activity; rhodopsin kinase activity; ATP binding

Biological Process: desensitization of G-protein coupled receptor protein signaling pathway; G-protein coupled receptor internalization; receptor internalization; signal transduction; protein amino acid phosphorylation