Typical Testing Data/Standard Curve (for reference only)
Sheep Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2C ELISA Kit | GRIN2C elisa kit
Sheep Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2C ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: GRIN2C ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GRIN2C antibody and an GRIN2C-HRP conjugate. The assay sample and buffer are incubated together with GRIN2C-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GRIN2C concentration since GRIN2C from samples and GRIN2C-HRP conjugate compete for the anti-GRIN2C antibody binding site. Since the number of sites is limited, as more sites are occupied by GRIN2C from the sample, fewer sites are left to bind GRIN2C-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GRIN2C concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a subunit of the N-methyl-D-aspartate (NMDA) receptor, which is a subtype of ionotropic glutamate receptor. NMDA receptors are found in the central nervous system, are permeable to cations and have an important role in physiological processes such as learning, memory, and synaptic development. The receptor is a tetramer of different subunits (typically heterodimer of subunit 1 with one or more of subunits 2A-D), forming a channel that is permeable to calcium, potassium, and sodium, and whose properties are determined by subunit composition. Alterations in the subunit composition of the receptor are associated with pathophysiological conditions such as Parkinson's disease, Alzheimer's disease, depression, and schizophrenia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
Uniprot Description
NMDAR2C: NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2C/GRIN2C subfamily.
Protein type: Channel, calcium; Membrane protein, multi-pass; Membrane protein, integral; Channel, ligand-gated
Chromosomal Location of Human Ortholog: 17q25.1
Cellular Component: postsynaptic membrane; integral to plasma membrane; dendrite; postsynaptic density; plasma membrane; cell junction; N-methyl-D-aspartate selective glutamate receptor complex
Molecular Function: protein binding; extracellular-glutamate-gated ion channel activity; N-methyl-D-aspartate selective glutamate receptor activity; protein N-terminus binding; cation channel activity; PDZ domain binding
Biological Process: synaptic transmission, glutamatergic; synaptic transmission; protein localization; directional locomotion; glutamate signaling pathway; transport; ionotropic glutamate receptor signaling pathway; response to wounding; negative regulation of protein catabolic process; regulation of excitatory postsynaptic membrane potential; neuromuscular process controlling balance
Research Articles on GRIN2C
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Product Notes
The Sheep GRIN2C grin2c (Catalog #AAA749506) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA749506 ELISA Kit recognizes Sheep GRIN2C. It is sometimes possible for the material contained within the vial of "Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2C, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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