Human Probable G-protein coupled receptor 146 ELISA Kit | GPR146 elisa kit
Human Probable G-protein coupled receptor 146 (GPR146) ELISA Kit
Gene Names
GPR146; PGR8
Reactivity
Human
Synonyms
Probable G-protein coupled receptor 146; Human Probable G-protein coupled receptor 146 (GPR146) ELISA Kit; GPR146 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human GPR146. No significant cross-reactivity or interference between Human GPR146 and analogues was observed.
Sequence Length
333
Samples
Serum, plasma, tissue homogenates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156 ng/mL - 10 ng/mL
Sensitivity
0.078 ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for GPR146 elisa kit
Intended Uses: For the quantitative detection of Human Probable G-protein coupled receptor 146 (GPR146) concentration in serum, plasma, tissue homogenates and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GPR146 in Human serum, plasma, tissue homogenates. An antibody specific for GPR146 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GPR146 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GPR146 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPR146 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GPR146 in Human serum, plasma, tissue homogenates. An antibody specific for GPR146 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GPR146 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GPR146 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPR146 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,580 Da
NCBI Official Full Name
probable G-protein coupled receptor 146
NCBI Official Synonym Full Names
G protein-coupled receptor 146
NCBI Official Symbol
GPR146
NCBI Official Synonym Symbols
PGR8
NCBI Protein Information
probable G-protein coupled receptor 146
UniProt Protein Name
Probable G-protein coupled receptor 146
Protein Family
UniProt Gene Name
GPR146
UniProt Synonym Gene Names
PGR8
Uniprot Description
GPR146: Orphan receptor. Belongs to the G-protein coupled receptor 1 family.
Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR
Chromosomal Location of Human Ortholog: 7p22.3
Cellular Component: integral component of membrane; plasma membrane
Molecular Function: G-protein coupled receptor activity
Biological Process: G-protein coupled receptor signaling pathway
Research Articles on GPR146
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Product Notes
The Human GPR146 gpr146 (Catalog #AAA283547) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA283547 ELISA Kit recognizes Human GPR146. It is sometimes possible for the material contained within the vial of "Probable G-protein coupled receptor 146, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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