GPR133 Recombinant Protein | ADGRD1 recombinant protein

GPR133 Protein, Human, Recombinant (ECD, His Tag)

Cat. Num. AAA8121976

• Gene Names: GPR133; PGR25
• Purity: >90% as determined by SDS-PAGE
• Synonyms: GPR133; GPR133 Protein; Human; Recombinant (ECD; His Tag); Human GPR133/PGR25 Protein (ECD; PGR25 Protein; ADGRD1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser570

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Val 26
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human GPR133 (NP_942122.2)(Met1-Ser570) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ADGRD1 recombinant protein

Background: ADGRD1 (Adhesion G Protein-Coupled Receptor D1, also known as GPR133) is a Protein Coding gene. 4 alternatively spliced human isoforms have been reported. ADGRD1, an orphan member of the adhesion family of G-protein-coupled receptors, is a critical regulator of the response to hypoxia and tumor growth in Glioblastoma (GBM). ADGRD1 represents a novel molecular target in GBM and possibly other malignancies where hypoxia is fundamental to pathogenesis. Variations in the ADGRD1 locus are linked with differences in metabolism, human height, and heart frequency. ADGRD1 is a Gs protein-coupled receptor belonging to the class of adhesion GPCRs. The adhesion G-protein-coupled receptors (GPCRs), including GPR133, are membrane-bound proteins with long N termini containing multiple domains.

NCBI and Uniprot Product Information

• NCBI GI #: 45433552
• NCBI GeneID: 283383
• NCBI Accession #: NP_942122.2
• NCBI GenBank Nucleotide #: NM_198827.3
• UniProt Accession #: Q6QNK2; Q2M1L3; Q6ZMQ1; Q7Z7M2; Q86SM4
• Molecular Weight: 96,530 Da
• NCBI Official Full Name: probable G-protein coupled receptor 133
• NCBI Official Synonym Full Names: G protein-coupled receptor 133
• NCBI Official Symbol: GPR133
• NCBI Official Synonym Symbols: PGR25
• NCBI Protein Information: probable G-protein coupled receptor 133; G-protein coupled receptor PGR25
• UniProt Protein Name: Probable G-protein coupled receptor 133
• Protein Family: Adhesion G protein-coupled receptor
• UniProt Gene Name: GPR133
• UniProt Synonym Gene Names: PGR25
• UniProt Entry Name: GP133_HUMAN

NCBI Description

The adhesion G-protein-coupled receptors (GPCRs), including GPR133, are membrane-bound proteins with long N termini containing multiple domains. GPCRs, or GPRs, contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins (summary by Bjarnadottir et al., 2004 [PubMed 15203201]).[supplied by OMIM, Nov 2010]

Uniprot Description

GPR133: Orphan receptor. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; GPCR, family 2

Chromosomal Location of Human Ortholog: 12q24.33

Cellular Component: plasma membrane; integral to membrane

Molecular Function: G-protein coupled receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway