Rat G Protein Coupled Receptor 120 ELISA Kit | GPR120 elisa kit

Rat G Protein Coupled Receptor 120 ELISA Kit

Cat. Num. AAA102225

• Gene Names: FFAR4; GT01; PGR4; BMIQ10; GPR120; GPR129; O3FAR1
• Reactivity: Rat
• Synonyms: G Protein Coupled Receptor 120; Rat G Protein Coupled Receptor 120 ELISA Kit; GPR120 elisa kit

• Reactivity: Rat
• Specificity: No significant cross-reactivity or interference between Rat GPR120 and analogues was observed.
• Sequence Length: 361

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 3.12 ng/ml - 100 ng/ml.
• Sensitivity: 1.0 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated GPR120 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat GPR120 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GPR120 and GPR120 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GPR120 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) are less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for GPR120 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated GPR120 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat GPR120 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GPR120 and GPR120 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GPR120 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 72533526
• NCBI GeneID: 338557
• UniProt Accession #: Q5NUL3; Q495H1; Q5VY25; Q5VY26; Q7Z605; Q86SM7
• Molecular Weight: 42,241 Da
• NCBI Official Full Name: GPR120 protein
• NCBI Official Synonym Full Names: free fatty acid receptor 4
• NCBI Official Symbol: FFAR4
• NCBI Official Synonym Symbols: GT01; PGR4; BMIQ10; GPR120; GPR129; O3FAR1
• NCBI Protein Information: free fatty acid receptor 4; omega-3 fatty acid receptor 1; G-protein coupled receptor 120; G-protein coupled receptor 129; G-protein coupled receptor GT01; G-protein coupled receptor PGR4
• UniProt Protein Name: Free fatty acid receptor 4
• UniProt Gene Name: FFAR4
• UniProt Synonym Gene Names: GPR120; GPR129; O3FAR1; PGR4
• UniProt Entry Name: FFAR4_HUMAN

NCBI Description

This gene encodes a G protein-coupled receptor (GPR) which belongs to the rhodopsin family of GPRs. The encoded protein functions as a receptor for free fatty acids, including omega-3, and participates in suppressing anti-inflammatory responses and insulin sensitizing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

GPR120: Receptor for medium and long-chain free fatty acid (FAA). Signals via a G(q)/G(11)-coupled pathway. Acts as a receptor for omega-3 fatty acids and mediates robust anti- inflammatory effects particularly in macrophages and fat cells. The anti-inflammatory effects involve inhibition of TAK1 through a beta-arrestin 2 (ARRB2)/TAB1 dependent effect but independent of G(q)/G(11)-coupled pathway. Mediates potent insulin sensitizing and antidiabetic effects by repressing macrophage-induced tissue inflammation. May mediate the taste of fatty acids. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid-binding; GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q23.33

Cellular Component: endocytic vesicle; integral to plasma membrane; plasma membrane

Molecular Function: peptide binding; fatty acid binding; taste receptor activity

Biological Process: hormone secretion; G-protein coupled receptor protein signaling pathway; negative regulation of inflammatory response; negative regulation of cytokine secretion; detection of chemical stimulus involved in sensory perception of taste; cellular response to hormone stimulus; negative regulation of apoptosis

Disease: Body Mass Index Quantitative Trait Locus 10