Typical Testing Data/Standard Curve (for reference only)
Bovine G Protein Coupled Receptor ELISA Kit | GPCR elisa kit
Bovine G Protein Coupled Receptor ELISA Kit
Gene Names
GPCR; F14G24.19; F14G24_19; G protein coupled receptor; G-PROTEIN COUPLED RECEPTOR 2; GCR2; GPCR
Reactivity
Bovine
Synonyms
G Protein Coupled Receptor; Bovine G Protein Coupled Receptor ELISA Kit; GPCR elisa kit
Reactivity
Bovine
Specificity
This assay has high sensitivity and excellent specificity for detection of GPCR. No significant cross-reactivity or interference between GPCR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GPCR and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GPCR elisa kit
Intended Uses: This GPCR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine GPCR. This ELISA kit for research use only!
Principle of the Assay: GPCR ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GPCR antibody and an GPCR-HRP conjugate. The assay sample and buffer are incubated together with GPCR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GPCR concentration since GPCR from samples and GPCR-HRP conjugate compete for the anti-GPCR antibody binding site. Since the number of sites is limited, as more sites are occupied by GPCR from the sample, fewer sites are left to bind GPCR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPCR concentration in each sample is interpolated from this standard curve.
Principle of the Assay: GPCR ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GPCR antibody and an GPCR-HRP conjugate. The assay sample and buffer are incubated together with GPCR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GPCR concentration since GPCR from samples and GPCR-HRP conjugate compete for the anti-GPCR antibody binding site. Since the number of sites is limited, as more sites are occupied by GPCR from the sample, fewer sites are left to bind GPCR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPCR concentration in each sample is interpolated from this standard curve.
Product Categories/Family for GPCR elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,451 Da
NCBI Official Full Name
G-protein coupled receptor 2
NCBI Official Symbol
GPCR
NCBI Official Synonym Symbols
F14G24.19; F14G24_19; G protein coupled receptor; G-PROTEIN COUPLED RECEPTOR 2; GCR2; GPCR
NCBI Protein Information
G-protein coupled receptor 2
UniProt Protein Name
LanC-like protein GCR2
UniProt Gene Name
GCR2
UniProt Synonym Gene Names
GPCR; At1g52920; F14G24.19
UniProt Entry Name
GCR2_ARATH
NCBI Description
Encodes a plasma membrane localized ABA receptor, which interacts with the Gαβγ complex. It has been postulated that the binding of ABA to GCR2 results in the release of the G protein and dissociation of the heterotrimeric complex into Gα and the Gβγ dimer to activate downstream ABA effectors and to trigger the ABA responses.
Uniprot Description
May play a role in abscisic acid (ABA) signaling.
Research Articles on GPCR
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Product Notes
The Bovine GPCR gcr2 (Catalog #AAA739616) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA739616 ELISA Kit recognizes Bovine GPCR. It is sometimes possible for the material contained within the vial of "G Protein Coupled Receptor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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