Human glypican 6 ELISA Kit | GPC6 elisa kit

Human glypican 6, GPC6 ELISA Kit

Cat. Num. AAA903155

• Gene Names: GPC6; OMIMD1
• Reactivity: Human
• Synonyms: glypican 6; Human glypican 6; GPC6 ELISA Kit; Human glypican 6 (GPC6) ELISA kit; MGC126288; OMIMD1; glypican proteoglycan 6; GPC6 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human GPC6. No significant cross-reactivity or interference between human GPC6 and analogues was observed.
• Sequence Length: 555

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 0. 312 ng/ml -20 ng/ml.
• Sensitivity: The minimum detectable dose of human GPC6 is typically less than 0.078 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GPC6 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GPC6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GPC6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GPC6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPC6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 5031719
• NCBI GeneID: 10082
• NCBI Accession #: NP_005699.1
• NCBI GenBank Nucleotide #: NM_005708.3
• UniProt Accession #: Q9Y625; Q96SG5; Q96SG8; Q9H1P4; A8K279
• Molecular Weight: 62,736 Da
• NCBI Official Full Name: glypican-6
• NCBI Official Synonym Full Names: glypican 6
• NCBI Official Symbol: GPC6
• NCBI Official Synonym Symbols: OMIMD1
• NCBI Protein Information: glypican-6; glypican proteoglycan 6
• UniProt Protein Name: Glypican-6
• Protein Family: Glypican
• UniProt Gene Name: GPC6
• UniProt Synonym Gene Names: UNQ369/PRO705
• UniProt Entry Name: GPC6_HUMAN

NCBI Description

The glypicans comprise a family of glycosylphosphatidylinositol-anchored heparan sulfate proteoglycans, and they have been implicated in the control of cell growth and cell division. The glypican encoded by this gene is a putative cell surface coreceptor for growth factors, extracellular matrix proteins, proteases and anti-proteases. [provided by RefSeq, Jan 2009]

Uniprot Description

GPC6: Cell surface proteoglycan that bears heparan sulfate. Putative cell surface coreceptor for growth factors, extracellular matrix proteins, proteases and anti-proteases. Enhances migration and invasion of cancer cells through WNT5A signaling. Defects in GPC6 are a cause of omodysplasia type 1 (OMOD1). OMOD1 is a rare autosomal recessive skeletal dysplasia characterized by severe congenital micromelia with shortening and distal tapering of the humeri and femora to give a club-like appearance. Typical facial features include a prominent forehead, frontal bossing, short nose with a depressed broad bridge, short columella, anteverted nostrils, long philtrum, and small chin. Point mutations leading to protein truncation, as well as larger genomic rearrangements resulting in exon deletions, have been found in family segregating omodysplasia type 1. All mutations identified in individuals affected by omodysplasia could lead to the absence of a functional protein, the mutant RNAs being suspected to be nonsense-mediated mRNA decay (NMD) targets. Even if the mRNA escapes NMD and is translated, all mutations are expected to disrupt the three-dimensional protein structure and often to abolish multiple highly conserved cysteine residues. Belongs to the glypican family.

Protein type: Cell surface; Motility/polarity/chemotaxis; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 13q32

Cellular Component: proteinaceous extracellular matrix; lysosomal lumen; extracellular space; Golgi lumen; integral to plasma membrane; plasma membrane; nucleus

Molecular Function: heparan sulfate proteoglycan binding

Biological Process: chondroitin sulfate metabolic process; phototransduction, visible light; glycosaminoglycan biosynthetic process; cell migration; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis; retinoid metabolic process

Disease: Omodysplasia 1