Glycoprotein IX Recombinant Protein | GP9 recombinant protein

Recombinant Glycoprotein IX, Platelet (GP9)

Cat. Num. AAA2030817

• Gene Names: GP9; GPIX; CD42a
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Glycoprotein IX; Recombinant Glycoprotein IX; Platelet (GP9); GP9 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MAPAQASQPI CWIPTWGLLL LLWPVTTATQ DCPRPCTCQS LETMGLKVNC EGRGLTALPV LPANTRQLLL TNNSLRSVPP GAFDHMPQLW ELKVAHNPWH CDCSLTYLRL WLEDHMPEALSHVYCASPDL ATQRPLGQLT GYELGNCGWK LPPSWAYSGI W
• Sequence Length: 1

• Applicable Applications for GP9 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 21.4kDa
• Accurate Molecular Mass (KD): 21kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag, T7 tag
• Organism Species: Rattus norvegicus (Rat)
• Fragment: Met1~Trp161
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4262781
• NCBI GeneID: 2815
• Molecular Weight: 19,046 Da
• NCBI Official Full Name: glycoprotein IX, partial
• NCBI Official Synonym Full Names: glycoprotein IX (platelet)
• NCBI Official Symbol: GP9
• NCBI Official Synonym Symbols: GPIX; CD42a
• NCBI Protein Information: platelet glycoprotein IX
• UniProt Protein Name: Platelet glycoprotein IX
• Protein Family: Platelet glycoprotein
• UniProt Gene Name: GP9
• UniProt Synonym Gene Names: GP-IX; GPIX
• UniProt Entry Name: GPIX_HUMAN

NCBI Description

This gene encodes a small membrane glycoprotein found on the surface of human platelets. It forms a 1-to-1 noncovalent complex with glycoprotein Ib, a platelet surface membrane glycoprotein complex that functions as a receptor for von Willebrand factor. The complete receptor complex includes noncovalent association of the alpha and beta subunits with the protein encoded by this gene and platelet glycoprotein V. Defects in this gene are a cause of Bernard-Soulier syndrome, also known as giant platelet disease. These patients have unusually large platelets and have a clinical bleeding tendency. [provided by RefSeq, Oct 2008]

Uniprot Description

GPIX: The GPIb-V-IX complex functions as the vWF receptor and mediates vWF-dependent platelet adhesion to blood vessels. The adhesion of platelets to injured vascular surfaces in the arterial circulation is a critical initiating event in hemostasis. GP-IX may provide for membrane insertion and orientation of GP-Ib. Defects in GP9 are a cause of Bernard-Soulier syndrome (BSS); also known as giant platelet disease (GPD). BSS patients have unusually large platelets and have a clinical bleeding tendency.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: integral to plasma membrane; plasma membrane

Biological Process: platelet activation; cell adhesion; blood coagulation; blood coagulation, intrinsic pathway

Disease: Bernard-soulier Syndrome