CD42c Recombinant Protein | GP1BB recombinant protein

CD42c Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120459

• Gene Names: GP1BB; BS; CD42C; GPIBB; BDPLT1
• Purity: >97% as determined by SDS-PAGE
• Synonyms: CD42c; CD42c Protein; Human; Recombinant (His Tag); Human GP1BB/CD42c Protein (His Tag); BDPLT1 Protein; BS Protein; CD42C Protein; GPIBB Protein; glycoprotein Ib (platelet); beta polypeptide; GP1BB recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Cys147

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Pro 27
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human GPIb, beta polypeptide extracellular domain (NP_000398.1)(Met 1-Cys 147) with a C-terminal polyhistidine tag was expressed.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for GP1BB recombinant protein

Background: Platelet glycoprotein Ib (GPIb) complex is best known as a major platelet receptor for von Willebrand factor essential for platelet adhesion under high shear conditions found in arteries and thrombosis. The GPIb complex is composed of GPIb alpha (Platelet glycoprotein Ib alpha chain) covalently attached to GPIb beta (Platelet glycoprotein Ib beta chain) and non-covalently complexed with GPIX and GPV. GPIb-beta, also known as GP1BB, CD42b-beta, and CD42c, is a single-pass type I membrane protein expressed in the heart and brain, which is a critical component of the von Willebrand factor (vWF) receptor. The cysteine knot region of GPIb beta in the N terminus is critical for the conformation of GPIb beta that interacts with GPIX. The precursor of GP1BB is synthesized from a 1.0 kb mRNA expressed in platelets and megakaryocytes. GPIb is a heterodimeric transmembrane protein consisting of a disulfide-linked 140 kD alpha chain and 22 kD beta chain. GPIb alpha chain provides the vWF binding site, and GPIb beta chain contributes to surface expression of the receptor and participates in transmembrane signaling through phosphorylation of its intracellular domain. GP1BB is part of the GPIb-V-IX system that constitutes the receptor for von Willebrand factor (vWF) and mediates platelet adhesion in the arterial circulation. Defects in GP1BB are a cause of Bernard-Soulier syndrome (BSS), also known as giant platelet disease (GPD). BSS patients have unusually large platelets and have a clinical bleeding tendency.

References

Kenny D, et al. (2002) The cysteine knot of platelet glycoprotein Ib beta (GPIb beta) is critical for the interaction of GPIb beta with GPIX. Blood. 99(12): 4428-33.Tang J,et al. (2004) Mutation in the leucine-rich repeat C-flanking region of platelet glycoprotein Ib beta impairs assembly of von Willebrand factor receptor. Thromb Haemost. 92(1): 75-88.Vanhoorelbeke K, et al. (2007) Inhibition of platelet glycoprotein Ib and its antithrombotic potential. Curr Pharm Des. 13(26): 2684-97.Clemetson KJ, et al. (2008) Platelet GPIb complex as a target for anti-thrombotic drug development. Thromb Haemost. 99(3): 473-9.

NCBI and Uniprot Product Information

• NCBI GI #: 4504073
• NCBI GeneID: 2812
• NCBI Accession #: NP_000398.1
• NCBI GenBank Nucleotide #: NM_000407.4
• UniProt Accession #: P13224; Q14422; Q8NG40
• Molecular Weight: 43,158 Da
• NCBI Official Full Name: platelet glycoprotein Ib beta chain
• NCBI Official Synonym Full Names: glycoprotein Ib (platelet), beta polypeptide
• NCBI Official Symbol: GP1BB
• NCBI Official Synonym Symbols: BS; CD42C; GPIBB; BDPLT1
• NCBI Protein Information: platelet glycoprotein Ib beta chain; GP-Ib beta; antigen CD42b-beta; nuclear localization signal deleted in velocardiofacial syndrome
• UniProt Protein Name: Platelet glycoprotein Ib beta chain
• Protein Family: Platelet glycoprotein
• UniProt Gene Name: GP1BB
• UniProt Synonym Gene Names: GP-Ib beta; GPIb-beta; GPIbB
• UniProt Entry Name: GP1BB_HUMAN

NCBI Description

Platelet glycoprotein Ib (GPIb) is a heterodimeric transmembrane protein consisting of a disulfide-linked 140 kD alpha chain and 22 kD beta chain. It is part of the GPIb-V-IX system that constitutes the receptor for von Willebrand factor (VWF), and mediates platelet adhesion in the arterial circulation. GPIb alpha chain provides the VWF binding site, and GPIb beta contributes to surface expression of the receptor and participates in transmembrane signaling through phosphorylation of its intracellular domain. Mutations in the GPIb beta subunit have been associated with Bernard-Soulier syndrome, velocardiofacial syndrome and giant platelet disorder. The 206 amino acid precursor of GPIb beta is synthesized from a 1.0 kb mRNA expressed in plateletes and megakaryocytes. A 411 amino acid protein arising from a longer, unspliced transcript in endothelial cells has been described; however, the authenticity of this product has been questioned. Yet another less abundant GPIb beta mRNA species of 3.5 kb, expressed in nonhematopoietic tissues such as endothelium, brain and heart, was shown to result from inefficient usage of a non-consensus polyA signal in the neighboring upstream gene (SEPT5, septin 5). In the absence of polyadenylation from its own imperfect site, the SEPT5 gene produces read-through transcripts that use the consensus polyA signal of this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

GPIbB: Gp-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to von Willebrand factor, which is already bound to the subendothelium. Defects in GP1BB are a cause of Bernard-Soulier syndrome (BSS); also known as giant platelet disease (GPD). BSS patients have unusually large platelets and have a clinical bleeding tendency. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding; transmembrane receptor activity

Biological Process: platelet activation; cell surface receptor linked signal transduction; blood coagulation; cell adhesion; blood coagulation, intrinsic pathway

Disease: Bernard-soulier Syndrome