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ICC (Immunocytochemistry) (ICC staining NGAL in SW480 cells (red). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Mouse anti-Human NGAL Monoclonal Antibody | anti-NGAL antibody

NGAL Antibody

Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry
Synonyms
NGAL; Monoclonal Antibody; NGAL Antibody; 24p3 antibody; 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody; 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody; Alpha 2 microglobulin related protein antibody; HGNC:6526 antibody; HNL antibody; Lcn 2 antibody; Lcn2 antibody; Lipocalin-2 antibody; Migration stimulating factor inhibitor antibody; MSFI antibody; Neutrophil gelatinase associated lipocalin antibody; Neutrophil gelatinase associated lipocalin precursor antibody; Neutrophil gelatinase-associated lipocalin antibody; NGAL antibody; NGAL_HUMAN antibody; Oncogene 24p3 antibody; Oncogenic lipocalin 24p3 antibody; p25 antibody; Siderocalin antibody; siderocalin LCN2 antibody; SV40 induced 24P3 protein antibody; Uterocalin antibody; anti-NGAL antibody
Ordering
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Clone Number
AH22-11
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.11% Sodium Azide.
Applicable Applications for anti-NGAL antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
Application Notes
WB: 1:1000
IHC: 1:50-1:200
ICC: 1:50-1:200
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

ICC (Immunocytochemistry)

(ICC staining NGAL in SW480 cells (red). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining NGAL in SW480 cells (red). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining NGAL in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining NGAL in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining NGAL in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining NGAL in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded huamn liver tissue using anti-NGAL antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded huamn liver tissue using anti-NGAL antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-NGAL antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-NGAL antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NGAL Tubulin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NGAL Tubulin antibody. Counter stained with hematoxylin.)
Related Product Information for anti-NGAL antibody
In addition to the monomeric mammalian progelatinase, two additional forms of progelatinase have been identified. The shorter of these additional forms is a covalently linked, disulfide-bridged protein that heterodimerizes with a short protein; an alpha2-Microglobulin-related protein known as neutrophil gelatinase-associated lipocalin (NGAL), which is moderately expressed in breast and lung tissues. NGAL belongs to the lipocalin family and has a high degree of similarity with rat alpha2-Microglobulin-related protein and mouse protein 24p3. NGAL is able to bind a derivative of the bacterial chemotactic peptide, suggesting that it has important immuno-modulatory functions. NGAL has been described as an inflammatory protein; it is released into the circulation as a result of the inflammatory activation of leukocytes initiated by the extra-corporeal circulation. In addition, NGAL synthesis is induced in epithelial cells in inflammatory and neoplastic colorectal diseases. In conclusion, NGAL may serve as a scavenger of bacterial products to function in the anti-inflammatory process.
Product Categories/Family for anti-NGAL antibody

NCBI and Uniprot Product Information

NCBI GI #
UniProt Accession #
Molecular Weight
22,588 Da
NCBI Official Full Name
LCN2
UniProt Protein Name
Neutrophil gelatinase-associated lipocalin
UniProt Gene Name
LCN2
UniProt Synonym Gene Names
HNL; NGAL; NGAL
UniProt Entry Name
NGAL_HUMAN

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Product Notes

The NGAL lcn2 (Catalog #AAA29895) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NGAL Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NGAL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC). WB: 1:1000 IHC: 1:50-1:200 ICC: 1:50-1:200. Researchers should empirically determine the suitability of the NGAL lcn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NGAL, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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