Typical Testing Data/Standard Curve (for reference only)
Human Golgi phosphoprotein 3 ELISA Kit | GOLPH3 elisa kit
Human Golgi phosphoprotein 3 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Golgi phosphoprotein 3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forGolgi phosphoprotein 3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Golgi phosphoprotein 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Golgi phosphoprotein 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a peripheral membrane protein of the Golgi stack and may have a regulatory role in Golgi trafficking. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
GOLPH3: Mediates the cis and medial Golgi localization of mannosyltransferases through direct binding of their cytosolic domains. Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene. Belongs to the GOLPH3/VPS74 family.
Protein type: Mitochondrial
Chromosomal Location of Human Ortholog: 5p13.3
Cellular Component: cytosol; Golgi apparatus; Golgi cisterna; intracellular membrane-bound organelle; mitochondrion; trans-Golgi network
Molecular Function: enzyme binding; protein binding
Biological Process: cell migration; cell proliferation; gene expression; glycoprotein biosynthetic process; Golgi organization and biogenesis; Golgi to plasma membrane protein transport; Golgi vesicle budding; lamellipodium biogenesis; leukocyte tethering or rolling; negative regulation of apoptosis; positive regulation of protein secretion; positive regulation of TOR signaling pathway; protein retention in Golgi; protein secretion; retrograde vesicle-mediated transport, Golgi to ER
Research Articles on GOLPH3
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Product Notes
The Human GOLPH3 golph3 (Catalog #AAA9427109) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9427109 ELISA Kit recognizes Human GOLPH3. It is sometimes possible for the material contained within the vial of "Golgi phosphoprotein 3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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