albumin Protein | ALB protein

Chromatographically purified goat albumin

Cat. Num. AAA570316

• Gene Names: ALB; PRO0883; PRO0903; PRO1341
• Reactivity: Caprine
• Applications: Immunodiffusion, ELISA, Western Blot
• Purity: Not less than 98% as determined by agar-gel electrophoresis. Tested in a concentration of 65 mg/ml at the level of sensitivity of the applied immunoprecipitating techniques, this product contains a trace of an alpha-1 protein.
• Synonyms: albumin; Chromatographically purified goat albumin; ALB protein

• Host: Goat serum
• Reactivity: Caprine
• Specificity: Chromatographically purified Goat albumin
• Purity/Purification: Not less than 98% as determined by agar-gel electrophoresis. Tested in a concentration of 65 mg/ml at the level of sensitivity of the applied immunoprecipitating techniques, this product contains a trace of an alpha-1 protein.
• Form/Format: Lyophilized, stable white powder. Vial with 100 mg goat albumin lyophilized from 2 ml PBS (pH 7.2).

• Applicable Applications for ALB protein: Immunodiffusion, ELISA (EIA), Western Blot (WB)
• Application Notes: Purified goat albumin may be used as a reference antigen, calibrator, coating protein and blocking agent in a variety of immunoassays including immunodiffusion, immuno¬elec¬trophoresis, ELISA, Western blotting, dot-immuno¬binding assays (DIBA), haemagglutination, and cell-binding assays
• Preservative: No preservative added.
• Preparation and Storage: This product is shipped lyophilized at ambient temperature. After arrival store at +4 degree C. Prolonged storage may be at -20 degree C. After opening the vial, the lyophilized content is reconstituted by adding 2 ml of sterile distilled water, mixed gently by inversion until complete dissolution is obtained. Allow to stand at ambient temperature for 5-10 minutes to reach equilibrium. Reconstituted solution may be stored frozen. Working dilutions may be prepared by adding the required amount of phosphate buffered saline (PBS), should not be refrozen and preferably used within 24 hours. Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product.

Related Product Information for ALB protein

Albumin is a stable small polypeptide with a strong antigenicity. It has a molecular weight of 69,000 and an isoelectric point of 5.7. The protein is isolated from serum by sequential precipitation and further purified by ion exchange chromatography and affinity chromatography. The identity is confirmed and tested by immunoelectrophoresis and radial immuno-diffusion using antisera to total serum proteins and albumin.

Product Categories/Family for ALB protein

Veterinary

NCBI and Uniprot Product Information

• NCBI GI #: 178344
• NCBI GeneID: 213
• UniProt Accession #: P02768; O95574; P04277; Q13140; Q645G4; Q68DN5; Q6UXK4; Q86YG0; Q9P157; Q9P1I7; Q9UHS3; Q9UJZ0
• Molecular Weight: 69,367 Da
• NCBI Official Full Name: albumin
• NCBI Official Synonym Full Names: albumin
• NCBI Official Symbol: ALB
• NCBI Official Synonym Symbols: PRO0883; PRO0903; PRO1341
• NCBI Protein Information: serum albumin; albumin (32 AA); albumin (AA 34); growth-inhibiting protein 20; cell growth inhibiting protein 42
• UniProt Protein Name: Serum albumin
• Protein Family: Albumin
• UniProt Gene Name: ALB
• UniProt Entry Name: ALBU_HUMAN

NCBI Description

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. [provided by RefSeq, Jul 2008]

Uniprot Description

albumin: Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Plasma. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Carrier

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; protein complex; extracellular region; basement membrane; nucleus

Molecular Function: antioxidant activity; toxin binding; protein binding; copper ion binding; enzyme binding; DNA binding; zinc ion binding; chaperone binding; drug binding; oxygen binding; fatty acid binding; pyridoxal phosphate binding

Biological Process: receptor-mediated endocytosis; platelet activation; sodium-independent organic anion transport; maintenance of mitochondrion localization; bile acid metabolic process; hemolysis by symbiont of host red blood cells; lipoprotein metabolic process; cellular response to starvation; response to mercury ion; response to organic substance; bile acid and bile salt transport; retinal homeostasis; platelet degranulation; transport; negative regulation of programmed cell death; blood coagulation; transmembrane transport; positive regulation of circadian sleep/wake cycle, non-REM sleep; response to nutrient; negative regulation of apoptosis

Disease: Analbuminemia; Hyperthyroxinemia, Familial Dysalbuminemic