Goat Anti-Bovine IgG(H+L) Secondary Antibody | IgG secondary antibody
Goat Anti-Bovine IgG(H+L)-BIOT
Applications
ELISA, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunoprecipitation, Immunoblot, Western Blot
Purity
Affinity chromatography on bovine IgG covalently linked to agarose
Synonyms
Goat Anti-Bovine IgG(H+L); Goat Anti-Bovine IgG(H+L)-BIOT; IgG secondary antibody
Host
Goat
Isotype
IgG
Specificity
Reacts with the heavy and light chains of bovine IgG
Purity/Purification
Affinity chromatography on bovine IgG covalently linked to agarose
Concentration
0.5 mg/mL (varies by lot)
Applicable Applications for IgG secondary antibody
ELISA (EIA), FLISA, Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunoblot (IB), Purification, Surface Plasmon Resonance
Application Notes
Quality tested applications for relevant formats include - ELISA; FLISA; Other referenced applications for relevant formats include -; Flow Cytometry; Immunocytochemistry; Immunoprecipitation; Immunoblotting; Purification; Surface Plasmon Resonance
Cross Adsorption
None; may react with immunoglobulins from other species and the light chains of other bovine immunoglobulins
Buffer Formulation
Supplied in PBS/NaN3
Isotype Control
Goat IgG-FITC
Preparation and Storage
Store at 2-8 degree C
References
1. Riedel M, Müller B, Wintermantel E. Protein adsorption and monocyte activation on germanium nanopyramids. Biomaterials. 2001;22:2307-16. (ELISA, Purification)
2. Liu J, Yu J, Wang M, Liu Q, Zhang W, Deng C, et al. Serodiagnosis of Neospora caninum infection in cattle using a recombinant tNcSRS2 protein-based ELISA. Vet Parasitol. 2007;143:358-63. (ELISA, WB)
3. Juleff N, Windsor M, Lefevre EA, Gubbins S, Hamblin P, Reid E, et al. Foot-and-mouth disease virus can induce a specific and rapid CD4+ T-cell-independent neutralizing and isotype class-switched antibody response in naïve cattle. J Virol. 2009;83:3626-36. (ELISA)
4. Cui J, Zhang B, Lin Y, Wang Q, Qian A, Nakane A, et al. Protective effect of glutathione S-transferase-fused mutant staphylococcal enterotoxin C against Staphylococcus aureus-induced bovine mastitis. Vet Immunol Immunopathol. 2010;135:64-70. (ELISA)
5. Otto W, Najnigier B, Stelmasiak T, Robins-Browne RM. Randomized control trials using a tablet formulation of hyperimmune bovine colostrum to prevent diarrhea caused by enterotoxigenic Escherichia coli in volunteers. Scand J Gastroenterol. 2011;46:862-8. (ELISA)
6. Mertens M, Vatansever Z, Mrenoshki S, Krstevski K, Stefanovska J, Djadjovski I, et al. Circulation of Crimean-Congo Hemorrhagic Fever Virus in the former Yugoslav Republic of Macedonia revealed by screening of cattle sera using a novel enzyme-linked immunosorbent assay. PLoS Negl Trop Dis. 2015;9(3):e0003519. (ELISA, ICC)
7. Guirnalda P, Murphy NB, Nolan D, Black SJ. Anti-Trypanosoma brucei activity in Cape buffalo serum during the cryptic phase of parasitemia is mediated by antibodies. Int J Pathol. 2007;37:1391-9. (FC)
8. Basso W, Lesser M, Grimm F, Hilbe M, Sydler T, Trösch L, et al. Bovine besnoitiosis in Switzerland: imported cases and local transmission. Vet Parasitol. 2013;198:265-73. (ICC)
9. Foucras G, Corbière F, Tasca C, Pichereaux C, Caubet C, Trumel C, et al. Alloantibodies against MHC class I: a novel mechanism of neonatal pancytopenia linked to vaccination. J Immunol. 2011;187:6564-70. (IP)
10. Odunuga OO, Shazhko A. Ammonium sulfate precipitation combined with liquid chromatography is sufficient for purification of bovine serum albumin that is suitable for most routine laboratory applications. Bio Chem Comp. 2013; 1:3 http://dx.doi.org/10.7243/2052-9341-1-3. (WB)
11. Indyk HE, Williams JW, Patel HA. Analysis of denaturation of bovine IgG by heat and high pressure using an optical biosensor. Int Dairy J. 2008;18:359-66. (Surface Plasmon Resonance)
2. Liu J, Yu J, Wang M, Liu Q, Zhang W, Deng C, et al. Serodiagnosis of Neospora caninum infection in cattle using a recombinant tNcSRS2 protein-based ELISA. Vet Parasitol. 2007;143:358-63. (ELISA, WB)
3. Juleff N, Windsor M, Lefevre EA, Gubbins S, Hamblin P, Reid E, et al. Foot-and-mouth disease virus can induce a specific and rapid CD4+ T-cell-independent neutralizing and isotype class-switched antibody response in naïve cattle. J Virol. 2009;83:3626-36. (ELISA)
4. Cui J, Zhang B, Lin Y, Wang Q, Qian A, Nakane A, et al. Protective effect of glutathione S-transferase-fused mutant staphylococcal enterotoxin C against Staphylococcus aureus-induced bovine mastitis. Vet Immunol Immunopathol. 2010;135:64-70. (ELISA)
5. Otto W, Najnigier B, Stelmasiak T, Robins-Browne RM. Randomized control trials using a tablet formulation of hyperimmune bovine colostrum to prevent diarrhea caused by enterotoxigenic Escherichia coli in volunteers. Scand J Gastroenterol. 2011;46:862-8. (ELISA)
6. Mertens M, Vatansever Z, Mrenoshki S, Krstevski K, Stefanovska J, Djadjovski I, et al. Circulation of Crimean-Congo Hemorrhagic Fever Virus in the former Yugoslav Republic of Macedonia revealed by screening of cattle sera using a novel enzyme-linked immunosorbent assay. PLoS Negl Trop Dis. 2015;9(3):e0003519. (ELISA, ICC)
7. Guirnalda P, Murphy NB, Nolan D, Black SJ. Anti-Trypanosoma brucei activity in Cape buffalo serum during the cryptic phase of parasitemia is mediated by antibodies. Int J Pathol. 2007;37:1391-9. (FC)
8. Basso W, Lesser M, Grimm F, Hilbe M, Sydler T, Trösch L, et al. Bovine besnoitiosis in Switzerland: imported cases and local transmission. Vet Parasitol. 2013;198:265-73. (ICC)
9. Foucras G, Corbière F, Tasca C, Pichereaux C, Caubet C, Trumel C, et al. Alloantibodies against MHC class I: a novel mechanism of neonatal pancytopenia linked to vaccination. J Immunol. 2011;187:6564-70. (IP)
10. Odunuga OO, Shazhko A. Ammonium sulfate precipitation combined with liquid chromatography is sufficient for purification of bovine serum albumin that is suitable for most routine laboratory applications. Bio Chem Comp. 2013; 1:3 http://dx.doi.org/10.7243/2052-9341-1-3. (WB)
11. Indyk HE, Williams JW, Patel HA. Analysis of denaturation of bovine IgG by heat and high pressure using an optical biosensor. Int Dairy J. 2008;18:359-66. (Surface Plasmon Resonance)
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Product Notes
The IgG (Catalog #AAA670655) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Goat Anti-Bovine IgG(H+L) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), FLISA, Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunoblot (IB), Purification, Surface Plasmon Resonance. Quality tested applications for relevant formats include - ELISA; FLISA; Other referenced applications for relevant formats include -; Flow Cytometry; Immunocytochemistry; Immunoprecipitation; Immunoblotting; Purification; Surface Plasmon Resonance. Researchers should empirically determine the suitability of the IgG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Goat Anti-Bovine IgG(H+L), Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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