Western Blot (WB)
((2ug/ml) staining of K562 nuclear cell lysate (A) + peptide (B) and negative control Human Hippocampus (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
Goat GATA1 Antibody | anti-GATA1 antibody
Goat Anti-GATA1 Antibody
Expected from sequence similarity: Human
Expected from sequence similarity: Human
IF: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10ug/ml.
FC/FACS: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.
Western Blot (WB)
((2ug/ml) staining of K562 nuclear cell lysate (A) + peptide (B) and negative control Human Hippocampus (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
Western Blot (WB)
((2ug/ml) staining of K562 nuclear cell lysate (A) + peptide (B) and negative control Human Hippocampus (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. [provided by RefSeq, Jul 2008]
Uniprot Description
GATA1: Transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. May form homodimers or heterodimers with other isoforms. Interacts (via the N-terminal zinc finger) with ZFPM1. Interacts with GFI1B. Interacts with PIAS4; the interaction enhances sumoylation and represses the transactivational activity in a sumoylation-independent manner. Interacts with LMCD1. Interacts with CREBBP; the interaction stimulates acetylation and transcriptional activity in vivo. Interacts with EP300. Erythrocytes. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: Xp11.23
Cellular Component: nucleoplasm; transcription factor complex; transcriptional repressor complex; nucleus
Molecular Function: protein binding; DNA binding; zinc ion binding; chromatin DNA binding; p53 binding; sequence-specific DNA binding; DNA bending activity; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; erythrocyte development; embryonic hemopoiesis; megakaryocyte differentiation; in utero embryonic development; positive regulation of erythrocyte differentiation; eosinophil differentiation; male gonad development; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; negative regulation of bone mineralization; positive regulation of peptidyl-tyrosine phosphorylation; cell-cell signaling; positive regulation of osteoblast proliferation; erythrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; platelet formation; blood coagulation; basophil differentiation; negative regulation of apoptosis
Disease: Thrombocytopenia With Beta-thalassemia, X-linked; Anemia, X-linked, With Or Without Neutropenia And/or Platelet Abnormalities; Thrombocytopenia, X-linked, With Or Without Dyserythropoietic Anemia; Down Syndrome
Research Articles on GATA1
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Product Notes
The GATA1 gata1 (Catalog #AAA423829) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-GATA1 Antibody reacts with Human Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's GATA1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), WB, Immunofluorescence (IF), Flow Cytometry (FC/FACS). Peptide ELISA: antibody detection limit dilution 1:32000 IF: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10ug/ml. FC/FACS: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml. Researchers should empirically determine the suitability of the GATA1 gata1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GATA1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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