GNMT recombinant protein

Recombinant GNMT protein

Cat. Num. AAA389346

• Synonyms: GNMT; Recombinant GNMT protein; Glycine N-Methyltransferase; GNMT recombinant protein

• Host: E Coli
• Form/Format: Recombinant GNMT protein is supplied in 25mM Tris-HCl pH8.0, 300mM NaCl, 10% glycerol and 0.5mM TCEP.

• Protein Species: Human
• Tag: His-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant GNMT protein gel 10% SDS-PAGE with Coomassie blue staining MW: 34.9 kDa Purity: >95%)

Related Product Information for GNMT recombinant protein

Short Description: Full-length human GNMT protein was expressed in E Coli (accession number NP_061833.1) with a C-terminal 6xHis-tag. The molecular weight of the protein is 34.9 kDa.

Background: GNMT (Glycine N-Methyltransferase) is an enzyme that catalyzes the conversion of S-adenosyl-L-methionine (along with glycine) to S-adenosyl-L-homocysteine and sarcosine. This protein is found in the cytoplasm and acts as a homo-tetramer. Defects in this gene are a cause of GNMT deficiency (hypermethioninemia). Alternative splicing results in multiple transcript variants.

Product Categories/Family for GNMT recombinant protein

Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 9506741
• NCBI GeneID: 27232
• NCBI Accession #: NP_061833.1
• NCBI GenBank Nucleotide #: NM_018960.4
• UniProt Accession #: Q14749; Q5T8W2; Q9NNZ1; Q9NS24
• Molecular Weight: 32,742 Da
• NCBI Official Full Name: glycine N-methyltransferase
• NCBI Official Synonym Full Names: glycine N-methyltransferase
• NCBI Official Symbol: GNMT
• NCBI Protein Information: glycine N-methyltransferase; HEL-S-182mP; epididymis secretory sperm binding protein Li 182mP
• UniProt Protein Name: Glycine N-methyltransferase
• Protein Family: Glycine N-methyltransferase
• UniProt Gene Name: GNMT
• UniProt Entry Name: GNMT_HUMAN

NCBI Description

The protein encoded by this gene is an enzyme that catalyzes the conversion of S-adenosyl-L-methionine (along with glycine) to S-adenosyl-L-homocysteine and sarcosine. The encoded protein is found in the cytoplasm and acts as a homotetramer. Defects in this gene are a cause of GNMT deficiency (hypermethioninemia). [provided by RefSeq, Oct 2008]

Uniprot Description

GNMT: Catalyzes the methylation of glycine by using S- adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine. Defects in GNMT are the cause of glycine N- methyltransferase deficiency (GNMT deficiency); also known as hypermethioninemia. The only clinical abnormalities in patients with this deficiency are mild hepatomegaly and chronic elevation of serum transaminases. Belongs to the class I-like SAM-binding methyltransferase superfamily. Glycine N-methyltransferase family.

Protein type: Amino Acid Metabolism - glycine, serine and threonine; Methyltransferase; EC 2.1.1.20

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: cytoplasm

Molecular Function: protein binding; glycine N-methyltransferase activity; glycine binding; folic acid binding

Biological Process: methylation; glycogen metabolic process; S-adenosylmethionine metabolic process; protein modification process; regulation of gluconeogenesis; methionine metabolic process; one-carbon compound metabolic process; protein homotetramerization

Disease: Glycine N-methyltransferase Deficiency