Rat Glucose Transporter 2 ELISA Kit | GLUT2 elisa kit

Rat Glucose Transporter 2 ELISA Kit

Cat. Num. AAA20970

• Gene Names: Slc2a2; Glut2; Glut-2; AI266973
• Reactivity: Rat
• Synonyms: Glucose Transporter 2; Rat Glucose Transporter 2 ELISA Kit; GLUT2 elisa kit

• Reactivity: Rat
• Specificity: No significant cross-reactivity or interference between Rat GLUT2 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 3.12 ng/ml - 100 ng/ml.
• Sensitivity: 1.0 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated GLUT2 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat GLUT2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GLUT2 and GLUT2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GLUT2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for GLUT2 elisa kit

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated GLUT2 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat GLUT2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GLUT2 and GLUT2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GLUT2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 683589
• NCBI GeneID: 20526
• UniProt Accession #: P14246; Q3TNA5; Q9DBA7
• Molecular Weight: 57,107 Da
• NCBI Official Full Name: GLUT2
• NCBI Official Synonym Full Names: solute carrier family 2 (facilitated glucose transporter), member 2
• NCBI Official Symbol: Slc2a2
• NCBI Official Synonym Symbols: Glut2; Glut-2; AI266973
• NCBI Protein Information: solute carrier family 2, facilitated glucose transporter member 2; liver-type glucose transporter; glucose transporter type 2, liver
• UniProt Protein Name: Solute carrier family 2, facilitated glucose transporter member 2
• Protein Family: Glucose transporter
• UniProt Gene Name: Slc2a2
• UniProt Synonym Gene Names: Glut-2; Glut2; GLUT-2
• UniProt Entry Name: GTR2_MOUSE

Uniprot Description

GLUT2: an integral membrane facilitative glucose transporter. One of 13 members of the human equilibrative glucose transport protein family. Present at high levels in the plasma membrane of pancreatic b-cells where it initiates the first step in glucose-stimulated insulin secretion, a glucose metabolism-dependent signaling mechanism. May mediate the bidirectional transfer of glucose across the plasma membrane of hepatocytes. May also participate with the Na( +2)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney. Required for glucose sensitivity in the hypothalamus and brain stem. Involved in the control of food intake and stimulation of glucose uptake by peripheral tissues.

Protein type: Transporter; Membrane protein, integral; Transporter, SLC family; Membrane protein, multi-pass

Cellular Component: membrane; basolateral plasma membrane; brush border membrane; cytoplasm; integral to membrane; plasma membrane; cytosol; endosome; brush border

Molecular Function: D-glucose transmembrane transporter activity; dehydroascorbic acid transporter activity; transporter activity; glucose transmembrane transporter activity; transmembrane transporter activity; substrate-specific transmembrane transporter activity; insulin receptor binding

Biological Process: carbohydrate utilization; transport; carbohydrate transport; glucose transport; transmembrane transport