Mouse Glucose Transporter 1 ELISA Kit | GLUT1 elisa kit

Mouse Glucose Transporter 1 ELISA Kit

Cat. Num. AAA761261

• Gene Names: Glut1; 0074/12; CG1086; CG13908; CG43946; DmelCG43946; Dmglut1; DmGluT1; DmGLUT1; Glt1; GLU; Glu T1; Glut-1; glut1; l(3)61Ea; l(3)L1H; l(3)S007412
• Reactivity: Mouse
• Synonyms: Glucose Transporter 1; Mouse Glucose Transporter 1 ELISA Kit; GLUT1 (Glucose Transporter 1)/SLC2A1/Solute carrier family 2; facilitated glucose transporter member 1/Glucose transporter type 1; erythrocyte/brain/GLUT-1/HepG2 glucose transporter/GLUT1/PED/DYT9/GLUT/DYT17/DYT18/EIG12/GLUT1DS; GLUT1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of GLUL. No significant cross-reactivity or interference between GLUL and analogues was observed.
• Sequence Length: 548

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 15.6-1000pg/ml
• Sensitivity: <9.375pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GLUL were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GLUL were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GLUT1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-GLUL antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-GLUL antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GLUL amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GLUL can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 665409623
• NCBI GeneID: 38109
• NCBI Accession #: NP_001286896.1
• NCBI GenBank Nucleotide #: NM_001299967.1
• Molecular Weight: 16,880 Da
• NCBI Official Full Name: glucose transporter 1, isoform Z
• NCBI Official Synonym Full Names: Glucose transporter 1
• NCBI Official Symbol: Glut1
• NCBI Official Synonym Symbols: 0074/12; CG1086; CG13908; CG43946; DmelCG43946; Dmglut1; DmGluT1; DmGLUT1; Glt1; GLU; Glu T1; Glut-1; glut1; l(3)61Ea; l(3)L1H; l(3)S007412
• NCBI Protein Information: CG43946 gene product from transcript CG43946-RR
• UniProt Protein Name: Glucose transporter type 1
• Protein Family: Glucose transporter
• UniProt Gene Name: Glut1
• UniProt Entry Name: GTR1_DROME

Uniprot Description

Facilitative glucose transporter.