Glutamyl-Q tRNA (Asp) synthetase Recombinant Protein | gluQ recombinant protein
Recombinant Escherichia coli Glutamyl-Q tRNA (Asp) synthetase
NCBI and Uniprot Product Information
NCBI Description
A downstream start site at M10 is also possible; both starts have been used to overproduce active protein (Dubois, 2004; Campanacci, 2004). [More information is available at EcoGene: EG11362]. Glutamyl-Q tRNA(Asp) synthetase (Glu-Q-RS) catalyzes the addition of a glutamate residue to the queosine-modified wobble base of tRNA(Asp). [More information is available at EcoCyc: EG11362].
Uniprot Description
Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5-dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in position 34 of the tRNA(Asp), the wobble position of the QUC anticodon. Does not transfer glutamate to either tRNA(Glu) or tRNA(Gln). The incapacity of the glutamylated tRNA(Asp) to bind elongation factor Tu suggests that it is not involved in ribosomal protein biosynthesis.
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Product Notes
The gluQ gluq (Catalog #AAA1132103) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-308, full length protein. The amino acid sequence is listed below: MLPPYFLFKE MTDTQYIGRF APSPSGELHF GSLIAALGSY LQARARQGRW LVRIEDIDPP REVPGAAETI LRQLEHYGLH WDGDVLWQSQ RHDAYREALA WLHEQGLSYY CTCTRARIQS IGGIYDGHCR VLHHGPDNAA VRIRQQHPVT QFTDQLRGII HADEKLARED FIIHRRDGLF AYNLAVVVDD HFQGVTEIVR GADLIEPTVR QISLYQLFGW KVPDYIHLPL ALNPQGAKLS KQNHAPALPK GDPRPVLIAA LQFLGQQAEA HWQDFSVEQI LQSAVKNWRL TAVPESAIVN STFSNASC. It is sometimes possible for the material contained within the vial of "Glutamyl-Q tRNA (Asp) synthetase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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