Rat Glycolipid transfer protein domain-containing protein 1 ELISA Kit | GLTPD1 elisa kit

Rat Glycolipid transfer protein domain-containing protein 1 (GLTPD1) ELISA Kit

Cat. Num. AAA283842

• Gene Names: Cptp; Gltpd1
• Reactivity: Rat
• Synonyms: Glycolipid transfer protein domain-containing protein 1; Rat Glycolipid transfer protein domain-containing protein 1 (GLTPD1) ELISA Kit; MGC10334;; GLTPD1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat GLTPD1. No significant cross-reactivity or interference between Rat GLTPD1 and analogues was observed.
• Sequence Length: 211

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for GLTPD1 elisa kit

GLTPD1, Belongs to the GLTP family. GLTP is similar to bovine and porcine proteins which accelerate transfer of certain glycosphingolipids and glyceroglycolipids between membranes. It is thought to be a cytoplasmic protein.Detected in fibroblasts (at protein level). Detected in fibroblasts and in various cancer cell lines.
Glycolipid transfer protein is a cytosolic protein that catalyses the transfer of glycolipids between different intracellular membranes. This protein transport primarily different glycosphingolipids and glyceroglycolipids between intracellular membranes, but not phospholipids. It might be also involved in translocation of glucosylceramides. It was found in brain, kidney, spleen, lung, cerebellum, liver and heart.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GLTPD1 in samples. An antibody specific for GLTPD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGLTPD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GLTPD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GLTPD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 56090387
• NCBI GeneID: 313771
• NCBI Accession #: NP_001007704.1
• NCBI GenBank Nucleotide #: NM_001007703.1
• UniProt Accession #: Q5XIS2
• Molecular Weight: 24,522 Da
• NCBI Official Full Name: ceramide-1-phosphate transfer protein
• NCBI Official Synonym Full Names: ceramide-1-phosphate transfer protein
• NCBI Official Symbol: Cptp
• NCBI Official Synonym Symbols: Gltpd1
• NCBI Protein Information: ceramide-1-phosphate transfer protein
• UniProt Protein Name: Ceramide-1-phosphate transfer protein
• UniProt Gene Name: Cptp
• UniProt Synonym Gene Names: Gltpd1; CPTP

Uniprot Description

Mediates the intracellular transfer of ceramide-1-phosphate between organelle membranes and the cell membrane. Required for normal structure of the Golgi stacks. Can bind phosphoceramides with a variety of aliphatic chains, but has a preference for lipids with saturated C16:0 or monounsaturated C18:1 aliphatic chains, and is inefficient with phosphoceramides containing lignoceryl (C24:0). Plays a role in the regulation of the cellular levels of ceramide-1-phosphate, and thereby contributes to the regulation of phospholipase PLA2G4A activity and the release of arachidonic acid. Has no activity with galactosylceramide, lactosylceramide, sphingomyelin, phosphatidylcholine, phosphatidic acid and ceramide ().