Rat Glycine receptor subunit alpha-2 ELISA Kit | GLRA2 elisa kit
Rat Glycine receptor subunit alpha-2 (GLRA2) ELISA Kit
Gene Names
Glra2; RNNEOGLY
Reactivity
Rat
Synonyms
Glycine receptor subunit alpha-2; Rat Glycine receptor subunit alpha-2 (GLRA2) ELISA Kit; GLR; Glycine receptor; alpha-2 polypeptide|glycine receptor alpha 2 subunit; GLRA2 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat GLRA2. No significant cross-reactivity or interference between Rat GLRA2 and analogues was observed.
Sequence Length
452
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for GLRA2 elisa kit
The GLRA2 gene encodes the alpha-2 subunit of the glycine receptor. The alpha-1 subunit is encoded by the GLRA1 gene, which maps to chromosome 5q32. The GLRA2 gene is expressed in fetal brain and spinal cord, and the GLRA1 gene is expressed predominantly in adult brain and spinal cord .
Grenningloh et al. (1987) cloned and sequenced cDNAs of the strychnine-binding subunit of the rat glycine receptor, a neurotransmitter-gated chloride channel protein of the central nervous system. They found that the deduced polypeptide shows significant structural and amino acid sequence homology with nicotinic acetylcholine receptor proteins, indicating that they belong to a family of genes encoding neurotransmitter-gated ion channels.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GLRA2 in samples. An antibody specific for GLRA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGLRA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GLRA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GLRA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Grenningloh et al. (1987) cloned and sequenced cDNAs of the strychnine-binding subunit of the rat glycine receptor, a neurotransmitter-gated chloride channel protein of the central nervous system. They found that the deduced polypeptide shows significant structural and amino acid sequence homology with nicotinic acetylcholine receptor proteins, indicating that they belong to a family of genes encoding neurotransmitter-gated ion channels.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GLRA2 in samples. An antibody specific for GLRA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGLRA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GLRA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GLRA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52,037 Da
NCBI Official Full Name
glycine receptor subunit alpha-2
NCBI Official Synonym Full Names
glycine receptor, alpha 2
NCBI Official Symbol
Glra2
NCBI Official Synonym Symbols
RNNEOGLY
NCBI Protein Information
glycine receptor subunit alpha-2
UniProt Protein Name
Glycine receptor subunit alpha-2
Protein Family
UniProt Gene Name
Glra2
NCBI Description
glycine gated chloride channel; displays strychnine-sensitive ion channel activity; may play a role in synaptogenesis [RGD, Feb 2006]
Uniprot Description
Glycine receptors are ligand-gated chloride channels. Channel opening is triggered by extracellular glycine. Channel opening is also triggered by taurine and beta-alanine (PubMed:2176511). Plays a role in the down-regulation of neuronal excitability. Contributes to the generation of inhibitory postsynaptic currents. Plays a role in cellular responses to ethanol.
Research Articles on GLRA2
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Product Notes
The Rat GLRA2 glra2 (Catalog #AAA285032) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA285032 ELISA Kit recognizes Rat GLRA2. It is sometimes possible for the material contained within the vial of "Glycine receptor subunit alpha-2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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