Glucagon-Like Peptide 2 Receptor Assay System | GLP2R assay system
Glucagon-Like Peptide 2 Receptor (GLP2R) ACTOne Stable Cell Line
Synonyms
Glucagon-Like Peptide 2 Receptor; Glucagon-Like Peptide 2 Receptor (GLP2R) ACTOne Stable Cell Line; GLP2R assay system
Specificity
Natural and recombinant human E-Cadherin
Sequence Length
553
Application Notes
cAMP dependent human GLP2R cell-based assay; cell-based high-throughput screening of human GLP2R inhibitors
Samples
Cell Culture Supernates, Serum, Plasma (Heparin), Saliva And Urine
Assay Type
Sandwich
Detection Range
156pg/ml-10000pg/ml
Sensitivity
< 15pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Product is shipped on dry ice. Storage: Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130 degrees C preferably in liquid nitrogen vapor, until ready for use.
Related Product Information for GLP2R assay system
Principle of the Assay: Human E-Cadherin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for E-Cadherin has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: D155-I707) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for E-Cadherin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human E-Cadherin amount of sample captured in plate!!Background/Introduction: E-Cadherin, also called Cadherin 1(CDH1),Uvomorulin or calcium-dependent ashesion protein, epithelial. E-cadherin is a Ca(2+)-dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1. It contains 16 exons and a 65-kb-long intron 2. E-cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas. E-cadherin plays a central part in the process of epithelial morphogenesis and acts as a strong invasion suppressor in experimental tumor cell systems. The standard product used in this kit is recombinant gene expression with the molecular mass of 120KDa.
Product Categories/Family for GLP2R assay system
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63,001 Da
NCBI Official Full Name
glucagon-like peptide 2 receptor
NCBI Official Synonym Full Names
glucagon-like peptide 2 receptor
NCBI Official Symbol
GLP2R
NCBI Protein Information
glucagon-like peptide 2 receptor; GLP-2 receptor
UniProt Protein Name
Glucagon-like peptide 2 receptor
Protein Family
UniProt Gene Name
GLP2R
UniProt Entry Name
GLP2R_HUMAN
NCBI Description
This gene encodes a G protein-coupled receptor that is closely related to the glucagon receptor and binds to glucagon-like peptide-2 (GLP2). Signalling through GLP2 stimulates intestinal growth and increases villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. [provided by RefSeq, Dec 2014]
Uniprot Description
GLP2R: This is a receptor for glucagon-like peptide 2. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Belongs to the G-protein coupled receptor 2 family.
Protein type: Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 2
Chromosomal Location of Human Ortholog: 17p13.3
Cellular Component: integral to membrane; plasma membrane
Molecular Function: G-protein coupled receptor activity; glucagon receptor activity
Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; positive regulation of cell proliferation
Research Articles on GLP2R
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Product Notes
The GLP2R glp2r (Catalog #AAA433622) is an Assay System and is intended for research purposes only. The product is available for immediate purchase. cAMP dependent human GLP2R cell-based assay; cell-based high-throughput screening of human GLP2R inhibitors. Researchers should empirically determine the suitability of the GLP2R glp2r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glucagon-Like Peptide 2 Receptor, Assay System" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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