Gamma-Glutamyl Hydrolase Recombinant Protein | gGH recombinant protein

Recombinant Gamma-Glutamyl Hydrolase (gGH)

Cat. Num. AAA2029533

• Gene Names: GGH; GH
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Gamma-Glutamyl Hydrolase; Recombinant Gamma-Glutamyl Hydrolase (gGH); gGH recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; RPHGDT AKKPIIGILM QKCRNKVMKNYGRYYIAASY VKYLESAGAR VVPVRLDLTE KDYEILFKSI NGILFPGGSVDLRRSDYAKV AKIFYNLSIQ SFDDGDYFPV WGTCLGFEEL SLLISGECLLTATDTVDVAM PLNFTGGQLH SRMFQNFPTE LLLSLAVEPL TANFHKWSLSVKNFTMNEKL KKFFNVLTTN TDGKIEFIST MEGYKYPVYG VQWHPEKAPYEWKNLDGISH APNAVKTAFY LAEFFVNEAR KNNHHFKSES EEEKALIYQFSPIYTGNISS FQQCYIFD
• Sequence Length: 318

• Applicable Applications for gGH recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 37.3kDa
• Accurate Molecular Mass (KD): 37kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and S-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Arg25~Asp318
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4503987
• NCBI GeneID: 8836
• NCBI Accession #: NP_003869.1
• NCBI GenBank Nucleotide #: NM_003878.2
• Molecular Weight: 35,964 Da
• NCBI Official Full Name: gamma-glutamyl hydrolase
• NCBI Official Synonym Full Names: gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase)
• NCBI Official Symbol: GGH
• NCBI Official Synonym Symbols: GH
• NCBI Protein Information: gamma-glutamyl hydrolase
• UniProt Protein Name: Gamma-glutamyl hydrolase
• Protein Family: Gamma-glutamyl hydrolase
• UniProt Gene Name: GGH
• UniProt Entry Name: GGH_HUMAN

NCBI Description

This gene catalyzes the hydrolysis of folylpoly-gamma-glutamates and antifolylpoly-gamma-glutamates by the removal of gamma-linked polyglutamates and glutamate. [provided by RefSeq, Jul 2008]

Uniprot Description

GGH: Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha- glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates. Belongs to the peptidase C26 family.

Protein type: Secreted; EC 3.4.19.9; Secreted, signal peptide; Cofactor and Vitamin Metabolism - folate biosynthesis; Protease

Chromosomal Location of Human Ortholog: 8q12.3

Cellular Component: extracellular space; lysosome; melanosome; nucleus; cytosol

Molecular Function: omega peptidase activity; exopeptidase activity

Biological Process: response to drug; response to ethanol; response to zinc ion; glutamine metabolic process; proteolysis; response to insulin stimulus