Glutamine Fructose-6-Phosphate Transaminase 2 Recombinant Protein | GFPT2 recombinant protein

Recombinant Glutamine Fructose-6-Phosphate Transaminase 2 (GFPT2)

Cat. Num. AAA2030334

• Gene Names: GFPT2; GFAT2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Glutamine Fructose-6-Phosphate Transaminase 2; Recombinant Glutamine Fructose-6-Phosphate Transaminase 2 (GFPT2); GFPT2 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; CGIFAYMNY RVPRTRKEIF ETLIKGLQRL EYRGYDSAGV AIDGNNHEVKERHIQLVKKR GKVKALDEEL YKQDSMDLKV EFETHFGIAH TRWATHGVPSAVNSHPQRSD KGNEFVVIHN GIITNYKDLR KFLESKGYEF ESETDTETIAKLIKYVFDNR ETEDITFSTL VERVIQQLEG AFALVFKSVH YPGEAVATRRGSPLLIGVRS KYKLSTEQIP ILYRTCTLEN VKNICKTRMK RLDSSACLHAVGDKAVEFFF ASDASAIIEH TNRVIFLEDD DIAAVADG
• Sequence Length: 682

• Applicable Applications for GFPT2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 36.4kDa
• Accurate Molecular Mass (KD): 36kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal tags, his-tag and T7 tag
• Organism Species: Homo sapiens (Human)
• Fragment: Cys2~Gly288
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 12652545
• NCBI GeneID: 9945
• Molecular Weight: 76,931 Da
• NCBI Official Full Name: Glutamine-fructose-6-phosphate transaminase 2
• NCBI Official Synonym Full Names: glutamine-fructose-6-phosphate transaminase 2
• NCBI Official Symbol: GFPT2
• NCBI Official Synonym Symbols: GFAT2
• NCBI Protein Information: glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2
• UniProt Protein Name: Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2
• Protein Family: Glutamine--fructose-6-phosphate aminotransferase
• UniProt Gene Name: GFPT2
• UniProt Synonym Gene Names: GFAT 2; GFAT2
• UniProt Entry Name: GFPT2_HUMAN

Uniprot Description

GFAT2: Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. Highest levels of expression in heart, placenta, and spinal cord.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Transferase; Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 2.6.1.16

Chromosomal Location of Human Ortholog: 5q34-q35

Cellular Component: cytosol

Molecular Function: carbohydrate binding; glutamine-fructose-6-phosphate transaminase (isomerizing) activity

Biological Process: cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; energy reserve metabolic process; UDP-N-acetylglucosamine biosynthetic process; glutamine metabolic process; protein amino acid N-linked glycosylation via asparagine; fructose 6-phosphate metabolic process; post-translational protein modification; carbohydrate biosynthetic process