Monkey Glial Fibrillary Acidic Protein ELISA Kit | GFAP elisa kit

Monkey Glial Fibrillary Acidic Protein ELISA Kit

Cat. Num. AAA734569

• Reactivity: Monkey
• Synonyms: Glial Fibrillary Acidic Protein; Monkey Glial Fibrillary Acidic Protein ELISA Kit; GFAP elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 2.5-50ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This GFAP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey GFAP. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GFAP elisa kit

Principle of the Assay: GFAP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GFAP. Standards or samples are then added to the microtiter plate wells and GFAP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GFAP present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for GFAP are added to each well to "sandwich" the GFAP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GFAP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GFAP concentration in each sample is interpolated from this standard curve.

Product Categories/Family for GFAP elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 251802
• NCBI GeneID: 2670
• Molecular Weight: 49,508 Da
• NCBI Official Full Name: glial fibrillary acidic protein
• NCBI Official Synonym Full Names: glial fibrillary acidic protein
• NCBI Official Symbol: GFAP
• NCBI Protein Information: glial fibrillary acidic protein
• UniProt Protein Name: Glial fibrillary acidic protein
• Protein Family: Glial fibrillary acidic protein
• UniProt Gene Name: GFAP
• UniProt Synonym Gene Names: GFAP
• UniProt Entry Name: GFAP_HUMAN

NCBI Description

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Uniprot Description

GFAP: a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: membrane; cytoplasm; intermediate filament; cytosol

Molecular Function: integrin binding; structural constituent of cytoskeleton; kinase binding

Biological Process: Bergmann glial cell differentiation; extracellular matrix organization and biogenesis; regulation of neurotransmitter uptake; response to wounding; neurite regeneration; intermediate filament organization; astrocyte development

Disease: Alexander Disease