General Sialic Acid (SA) ELISA Kit | SA elisa kit

General Sialic Acid (SA) ELISA Kit

Cat. Num. AAA2707420

• Gene Names: SIGLEC1; SA; SN; pSn; p210; SIGLEC-1
• Reactivity: General
• Synonyms: Sialic Acid (SA); General Sialic Acid (SA) ELISA Kit; SA elisa kit

• Reactivity: General
• Specificity: This assay has high sensitivity and excellent specificity for detection of Sialic Acid (SA).; No significant cross-reactivity or interference between Sialic Acid (SA) and analogues was observed.
• Sequence Length: 1730

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 246.9-20,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 89.4pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sialic Acid (SA) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sialic Acid (SA) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SA elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Sialic Acid (SA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Sialic Acid (SA) and unlabeled Sialic Acid (SA) (Standards or samples) with the pre-coated antibody specific to Sialic Acid (SA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Sialic Acid (SA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Sialic Acid (SA) in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 50979305
• NCBI GeneID: 397623
• NCBI GenBank Nucleotide #: NP_999511.1
• UniProt Accession #: A7LCJ3
• NCBI Official Full Name: sialoadhesin
• NCBI Official Symbol: SIGLEC1
• NCBI Official Synonym Symbols: SA; SN; pSn; p210; SIGLEC-1
• NCBI Protein Information: sialoadhesin
• UniProt Protein Name: Sialoadhesin
• Protein Family: Phosphoadenosine phosphosulfate reductase
• UniProt Gene Name: SIGLEC1
• UniProt Synonym Gene Names: SA; SN; pSn; Siglec-1
• UniProt Entry Name: SN_PIG

Uniprot Description

Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid. Binds to SPN/CD43 on T-cells. May play a role in hemopoiesis (). Acts as an endocytic receptor mediating clathrin dependent endocytosis. In case of porcine reproductive and respiratory syndrome virus (PRRSV), mediates virion attachment and internalization into alveolar macrophages through a clathrin-coated dependent process.