Typical Testing Data/Standard Curve (for reference only)
General Heat Stable Enterotoxin a (STa) ELISA Kit | STa elisa kit
General Heat Stable Enterotoxin a (STa) ELISA Kit
Reactivity
General
Synonyms
Heat Stable Enterotoxin a (STa); General Heat Stable Enterotoxin a (STa) ELISA Kit; STa elisa kit
Reactivity
General
Specificity
This assay has high sensitivity and excellent specificity for detection of STa. No significant cross-reactivity or interference between STa and analogues was observed.
Sequence Length
71
Application Notes
Enzyme-linked immunosorbent assay for Antigen Detection.
Samples
Biological agents
Assay Type
Quantitative Sandwich
Detection Range
0.78-50ng/mL
Sensitivity
< 0.31ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level STa were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level STa were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for STa elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of STa in biological agents.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to STa. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to STa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain STa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of STa in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to STa. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to STa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain STa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of STa in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
7,494 Da
NCBI Official Full Name
Heat-stable enterotoxin A
UniProt Protein Name
Heat-stable enterotoxin A
Protein Family
UniProt Gene Name
ystA
UniProt Synonym Gene Names
yst
Uniprot Description
Toxin which activates the particulate form of guanylate cyclase and increases cyclic GMP levels within the host intestinal epithelial cells.
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Product Notes
The General STa ysta (Catalog #AAA2105011) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2105011 ELISA Kit recognizes General STa. Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the STa ysta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Heat Stable Enterotoxin a (STa), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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