General Adenosine Triphosphate (ATP) ELISA Kit | ATP elisa kit

General Adenosine Triphosphate (ATP) ELISA Kit

Cat. Num. AAA2700081

• Reactivity: General
• Synonyms: Adenosine Triphosphate (ATP); General Adenosine Triphosphate (ATP) ELISA Kit; Adenosine-5'-Triphosphate; ATP elisa kit

• Reactivity: General
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATP. No significant cross-reactivity or interference between ATP and analogues was observed.
• Sequence Length: 55

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Quantitative Competitive
• Detection Range: 12.35-1,000ng/mL
• Sensitivity: < 4.33ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATP elisa kit

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of ATP in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to ATP has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled ATP and unlabeled ATP (Standards or samples) with the pre-coated antibody specific to ATP. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of ATP in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of ATP in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 1161208
• Molecular Weight: 6,523 Da
• NCBI Official Full Name: adenosine triphosphate subunit 8 (mitochondrion)
• UniProt Protein Name: ATP synthase protein 8
• UniProt Gene Name: MT-ATP8
• UniProt Synonym Gene Names: ATP8; ATPASE8; MTATP8

Uniprot Description

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain. Minor subunit located with subunit a in the membrane ().