3-Methoxytyramine (3-MT) ELISA Kit | 3-MT elisa kit

General species 3-Methoxytyramine (3-MT)(Competitive EIA) ELISA Kit

Cat. Num. AAA2709735

• Reactivity: General species
• Applications: ELISA
• Synonyms: 3-Methoxytyramine (3-MT); General species 3-Methoxytyramine (3-MT)(Competitive EIA) ELISA Kit; ELISA Kit for 3-Methoxytyramine (3-MT); 3-MT/3-Methoxytyramine; 3-Methoxy-4-Hydroxyphenethylamine; 3-MT elisa kit

• Reactivity: General species
• Specificity: Reactive with General species 3-Methoxytyramine (3-MT); This assay has high sensitivity and excellent specificity for detection of 3-Methoxytyramine (3-MT). No significant cross-reactivity or interference between 3-Methoxytyramine (3-MT) and analogues was observed.

• Applicable Applications for 3-MT elisa kit: ELISA (EIA)
• Application Notes: Enzyme-linked immunosorbent assay for Antigen Detection.
• Assay Type: Competitive ELISA
• Sample Types: serum, plasma and other biological fluids
• Detection Method: Competitive Inhibition
• Detection Range: 61.7-5,000pg/mL
• Sensitivity: 21.9pg/mL
• Assay Length: 2 h
• Sample Size: 50ul (Please refer to the manual for the specific data.)
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 3-Methoxytyramine (3-MT) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 3-Methoxytyramine (3-MT) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Assay Procedure Summary: 1. Prepare all reagents, samples and standards; 2. Add 50ul standard or sample to each well.And then add 50ul prepared Detection Reagent A immediately.Shake and mix. Incubate 1 hour at 37 degree C; 3. Aspirate and wash 3 times; 4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C ; 5. Aspirate and wash 5 times; 6. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C ; 7. Add 50ul Stop Solution. Read at 450 nm immediately.
• Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Glycated Albumin (GA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Glycated Albumin (GA) and unlabeled Glycated Albumin (GA)(Standards or samples) with the pre-coated antibody specific to Glycated Albumin (GA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Glycated Albumin (GA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Glycated Albumin (GA) in the sample.
• Preparation and Storage: Store at 4 degree C for 1 month/ -20 degree C for long-term (within shelf life).; The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)