Porcine GDP Dissociation Inhibitor 1 ELISA Kit | GDI1 elisa kit

Porcine GDP Dissociation Inhibitor 1 ELISA Kit

Cat. Num. AAA741444

• Gene Names: GDI1; 1A; GDIL; MRX41; MRX48; OPHN2; XAP-4; RABGD1A; RABGDIA
• Reactivity: Porcine
• Synonyms: GDP Dissociation Inhibitor 1; Porcine GDP Dissociation Inhibitor 1 ELISA Kit; GDI1 elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This GDI1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine GDI1. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This GDI1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine GDI1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GDI1 elisa kit

Principle of the Assay: GDI1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GDI1 antibody and an GDI1-HRP conjugate. The assay sample and buffer are incubated together with GDI1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GDI1 concentration since GDI1 from samples and GDI1-HRP conjugate compete for the anti-GDI1 antibody binding site. Since the number of sites is limited, as more sites are occupied by GDI1 from the sample, fewer sites are left to bind GDI1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GDI1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for GDI1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 49457546
• NCBI GeneID: 2664
• UniProt Accession #: P31150; P50394; Q6FG50; Q7Z2G6; Q7Z2G9; Q7Z2H5; Q7Z2I6
• Molecular Weight: 50,583 Da
• NCBI Official Full Name: GDI1, partial
• NCBI Official Synonym Full Names: GDP dissociation inhibitor 1
• NCBI Official Symbol: GDI1
• NCBI Official Synonym Symbols: 1A; GDIL; MRX41; MRX48; OPHN2; XAP-4; RABGD1A; RABGDIA
• NCBI Protein Information: rab GDP dissociation inhibitor alpha; GDI-1; protein XAP-4; rab GDI alpha; oligophrenin-2; mental retardation, X-linked 48; rab GDP-dissociation inhibitor, alpha; guanosine diphosphate dissociation inhibitor 1
• UniProt Protein Name: Rab GDP dissociation inhibitor alpha
• Protein Family: Guanosine nucleotide diphosphate dissociation inhibitor
• UniProt Gene Name: GDI1
• UniProt Synonym Gene Names: GDIL; OPHN2; RABGDIA; XAP4; Rab GDI alpha; GDI-1
• UniProt Entry Name: GDIA_HUMAN

NCBI Description

GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI1 is expressed primarily in neural and sensory tissues. Mutations in GDI1 have been linked to X-linked nonspecific mental retardation. [provided by RefSeq, Jul 2008]

Uniprot Description

GDI1: a GDP dissociation inhibitor protein expressed primarily in neural and sensory tissues. GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the Rab family, small GTP-binding proteins of the Ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from Rab proteins and release GDP from membrane-bound Rabs. Mutations have been linked to X-linked nonspecific mental retardation.

Protein type: G protein regulator, misc.; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: Golgi apparatus; neuron projection; protein complex; cytoplasm; midbody; cytosol

Molecular Function: protein binding; GDP-dissociation inhibitor activity; Rab GDP-dissociation inhibitor activity; Rab GTPase binding; GTPase activator activity

Biological Process: protein transport; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; negative regulation of axonogenesis; signal transduction; Rab protein signal transduction; response to calcium ion; positive regulation of GTPase activity

Disease: Mental Retardation, X-linked 41