SDS-Page
GDF-15 recombinant protein
GDF-15, 195-308aa, Human, His tag, E Coli
Gene Names
GDF15; PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
GDF-15; 195-308aa; Human; His tag; E Coli; Growth differentiation factor 15; GDF15; MIC-1; MIC1; NAG-1; PDF; PLAB; PTGFB; Growth differentiation factor 15 NSAID regulated protein 1; Placental TGF beta; GDF 15; Growth/differentiation factor 15; Macrophage inhibitory cytokine 1; MIC 1; NAG 1; NAG1; NRG 1; NRG1; NSAID; NSAID (nonsteroidal anti inflammatory drug) activated protein 1; Placental bone morphogenetic protein; Placental bone morphogenic protein; Prostate differentiation factor; PTGF beta; PTGFB.; GDF-15 recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 10mM Sodium citrate (pH 3.5) containing 10% glycerol
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMARA RNGDHCPLGP GRCCRLHTVR ASLEDLGWAD WVLSPREVQV TMCIGACPSQ FRAANMHAQI KTSLHRLKPD TVPAPCCVPA SYNPMVLIQK TDTGVSLQTY DDLLAKDCHC I
Sequence Length
308
Applicable Applications for GDF-15 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for GDF-15 recombinant protein
Growth differentiation factor 15 (GDF-15) is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. This protein is most abundant in the liver, with lower levels seen in some other tissues. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF-15 was expressed in E Coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Product Categories/Family for GDF-15 recombinant protein
References
Paralkar V.M, et al. (1998) J Biol Chem. 273:13760-13767.; Hsiao E, Koniaris L., et al. (2000) Mol Cell Biol 20 (10):3742-51. ;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16.7 kDa (151aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)
NCBI Official Full Name
growth/differentiation factor 15 preproprotein
NCBI Official Synonym Full Names
growth differentiation factor 15
NCBI Official Symbol
GDF15
NCBI Official Synonym Symbols
PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15
NCBI Protein Information
growth/differentiation factor 15
UniProt Protein Name
Growth/differentiation factor 15
UniProt Gene Name
GDF15
UniProt Synonym Gene Names
MIC1; PDF; PLAB; PTGFB; GDF-15; MIC-1; NAG-1; NRG-1
NCBI Description
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The protein is expressed in a broad range of cell types, acts as a pleiotropic cytokine and is involved in the stress response program of cells after cellular injury. Increased protein levels are associated with disease states such as tissue hypoxia, inflammation, acute injury and oxidative stress. [provided by RefSeq, Aug 2016]
Research Articles on GDF-15
Similar Products
Product Notes
The GDF-15 gdf15 (Catalog #AAA203555) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's GDF-15 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the GDF-15 gdf15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMARA RNGDHCPLGP GRCCRLHTVR ASLEDLGWAD WVLSPREVQV TMCIGACPSQ FRAANMHAQI KTSLHRLKPD TVPAPCCVPA SYNPMVLIQK TDTGVSLQTY DDLLAKDCHC I. It is sometimes possible for the material contained within the vial of "GDF-15, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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