Typical Testing Data/Standard Curve (for reference only)
Human growth differentiation factor 15 ELISA Kit | GDF15 elisa kit
Human growth differentiation factor 15, GDF15 ELISA Kit
Gene Names
GDF15; PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15
Reactivity
Human
Synonyms
growth differentiation factor 15; Human growth differentiation factor 15; GDF15 ELISA Kit; Human growth differentiation factor 15 (GDF15) ELISA Kit; GDF-15; MIC-1; MIC1; NAG-1; PDF; PLAB; PTGFB; NSAID (nonsteroidal anti-inflammatory drug) -activated protein 1; PTGF-beta; prostate differentiation factor; GDF15 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human GDF15. No significant cross-reactivity or interference between Human GDF15 and analogues was observed.
Sequence Length
308
Samples
Serum, plasma, tissue homogenates, urine
Detection Range
7.8 pg/ml-500 pg/ml
Sensitivity
1.95 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GDF15 elisa kit
Introduction: Growth differentiation factors (GDF) are a subfamily of proteins belonging to the transforming growth factor beta superfamily that have functions predominantly in development. GDF15 (also known as TGF PL, MIC-1, PDF, PLAB, and PTGFB) has a role in regulating inflammatory and apoptotic pathways during tissue injury and certain disease processes. GDF15 mRNA is most abundant in the liver, with lower levels seen in some other tissues. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GDF15. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for GDF15 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain GDF15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of GDF15 in the samples is then determined by comparing the O.D. of the samples to the standard curve
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GDF15. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for GDF15 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain GDF15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of GDF15 in the samples is then determined by comparing the O.D. of the samples to the standard curve
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,140 Da
NCBI Official Full Name
growth/differentiation factor 15
NCBI Official Synonym Full Names
growth differentiation factor 15
NCBI Official Symbol
GDF15
NCBI Official Synonym Symbols
PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15
NCBI Protein Information
growth/differentiation factor 15; NRG-1; PTGF-beta; placental TGF-beta; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; prostate differentiation factor; macrophage inhibitory cytokine 1; placental bone morphogenetic protein; NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1
UniProt Protein Name
Growth/differentiation factor 15
Protein Family
UniProt Gene Name
GDF15
UniProt Synonym Gene Names
MIC1; PDF; PLAB; PTGFB; GDF-15; MIC-1; NAG-1; NRG-1
UniProt Entry Name
GDF15_HUMAN
NCBI Description
Bone morphogenetic proteins (e.g., BMP9; MIM 605120) are members of the transforming growth factor-beta (see TGFB1; MIM 190180) superfamily and regulate tissue differentiation and maintenance. They are synthesized as precursor molecules that are processed at a dibasic cleavage site to release C-terminal domains containing a characteristic motif of 7 conserved cysteines in the mature protein.[supplied by OMIM, Oct 2009]
Uniprot Description
GDF15: disulfide-linked. Belongs to the TGF-beta family.
Protein type: Secreted; Secreted, signal peptide; Cytokine
Chromosomal Location of Human Ortholog: 19p13.11
Cellular Component: extracellular space; extracellular region
Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding
Biological Process: regulation of apoptosis; cell-cell signaling; regulation of MAPKKK cascade; transforming growth factor beta receptor signaling pathway; signal transduction; cell development
Research Articles on GDF15
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Product Notes
The Human GDF15 gdf15 (Catalog #AAA703930) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA703930 ELISA Kit recognizes Human GDF15. It is sometimes possible for the material contained within the vial of "growth differentiation factor 15, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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