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SDS-Page

Growth Differentiation Factor 10 Recombinant Protein | GDF10 recombinant protein

Recombinant Growth Differentiation Factor 10 (GDF10)

Gene Names
GDF10; BMP3B; BMP-3b
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
>95%
Synonyms
Growth Differentiation Factor 10; Recombinant Growth Differentiation Factor 10 (GDF10); GDF10 recombinant protein
Ordering
For Research Use Only!
Host
Host: E Coli
Source: Prokaryotic expression
Purity/Purification
>95%
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Concentration
9.2 (varies by lot)
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-MALAPPPRGL WQAKDISPIV KAARRDGELL LSAQLDSEER DPGVPRPSPY APYILVYAND LAISEPNSVA VTLQRYDPFP AGDPEPRAAP NNSADPRVRR AAQATGPLQD NELPGLDERP PRAHAQHFHK HQLWPSPFRA LKPRPGRKDR RKKGQEVFMA ASQVLDFDEK TMQKARRKQW DEPRVCSRRY LKVDFADIGW NEWIISPKSF DAYYCAGACE FPMPKIVRPS NHATIQSIVRAVGIIPGIPE PCCVPDKMNS LGVLFLDENR NVVLKVYPNM SVDTCACR
Sequence Length
478
Applicable Applications for GDF10 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Predicted Molecular Mass
36.2kDa
Accurate Molecular Mass (KD)
36kDa
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Expression System
Prokaryotic expression
Tag
two N-terminal Tags, His-tag and T7-tag
Organism Species
Homo sapiens (Human)
Fragment
Met1~Arg288
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
53,122 Da
NCBI Official Full Name
growth differentiation factor 10
NCBI Official Synonym Full Names
growth differentiation factor 10
NCBI Official Symbol
GDF10
NCBI Official Synonym Symbols
BMP3B; BMP-3b
NCBI Protein Information
growth/differentiation factor 10
UniProt Protein Name
Growth/differentiation factor 10
UniProt Gene Name
GDF10
UniProt Synonym Gene Names
GDF-10; BMP-3B; BIP
UniProt Entry Name
GDF10_HUMAN

NCBI Description

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice suggest that the protein encoded by this gene plays a role in skeletal morphogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

GDF10: is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice suggest that plays a role in skeletal morphogenesis. [provided by RefSeq, Jul 2008]

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q11.22

Cellular Component: extracellular space

Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: fat cell differentiation; regulation of apoptosis; transforming growth factor beta receptor signaling pathway; regulation of MAPKKK cascade; cell development; skeletal development; growth

Research Articles on GDF10

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Product Notes

The GDF10 gdf10 (Catalog #AAA2031241) is a Recombinant Protein produced from Host: E Coli Source: Prokaryotic expression and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Growth Differentiation Factor 10 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the GDF10 gdf10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHH H SSGLVPRGSH MASMTGGQQM GRGSEF-MAL APPPRGL WQAKDISPIV KAARRDGELL LSAQLDSEER DPGVPRPSPY APYILVYAND LAISEPNSVA VTLQRYDPFP AGDPEPRAAP NNSADPRVRR AAQATGPLQD NELPGLDERP PRAHAQHFHK HQLWPSPFRA LKPRPGRKDR RKKGQEVFMA ASQVLDFDEK TMQKARRKQW DEPRVCSRRY LKVDFADIGW NEWIISPKSF DAYYCAGACE FPMPKIVRPS NHATIQSIVR AVGIIPGIPE PCCVPDKMNS LGVLFLDENR NVVLKVYPNM SVDTCACR. It is sometimes possible for the material contained within the vial of "Growth Differentiation Factor 10, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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