Human Glyceraldehyde-3-phosphate dehydrogenase ELISA Kit | GAPDH elisa kit
Human Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) ELISA Kit
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
Uniprot Description
GAPDH: a multifunctional enzyme with both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities. A key glycolytic enzyme that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. An important enzyme for energy metabolism, and the production of ATP and pyruvate through anaerobic glycolysis in the cytoplasm. Additionally, it participates in apoptosis, membrane trafficking, iron metabolism, nuclear activities and receptor mediated cell signaling. Its subcellular localization changes reflecting its multiple activities. Is cytosolic, but is also localized in the membrane, the nucleus, polysomes, the ER and the Golgi. Participates in transcription, RNA transport, DNA replication and apoptosis. S-nitrosylation on Cys-152 following apoptotic stimulates its interaction with SIAH2, which in turn moderates its translocation into the nucleus. Mediates cysteine S-nitrosylation of nuclear target proteins including SIRT1, HDAC2 and DNA-PK. Deregulated in lung cancer, renal cancer, breast cancer, gastric cancer, glioma, liver cancer, colorectal cancer, melanoma, prostatic cancer, pancreatic cancer and bladder cancer. Its increased expression and enzymatic activity is associated with cell proliferation and tumorigenesis, Oxidative stress impairs GAPDH catalytic activity and leads to cellular aging and apoptosis. In experimental animal models, injection of GAPDH antagonists induces apoptosis and blocks Hep3B tumor progression, suggesting a therapeutic potential of targeting GAPDH in human hepatocellular carcinoma
Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 1.2.1.12; Oxidoreductase
Chromosomal Location of Human Ortholog: 12p13.31
Cellular Component: cytoplasm; cytosol; extracellular exosome; extracellular matrix; intracellular membrane-bound organelle; intracellular ribonucleoprotein complex; lipid droplet; membrane; microtubule cytoskeleton; nuclear membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; vesicle
Molecular Function: aspartic-type endopeptidase inhibitor activity; glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; identical protein binding; microtubule binding; NAD binding; NADP binding; protein binding
Biological Process: antimicrobial humoral immune response mediated by antimicrobial peptide; cellular response to interferon-gamma; defense response to fungus; gluconeogenesis; killing by host of symbiont cells; killing of cells of other organism; microtubule cytoskeleton organization; negative regulation of endopeptidase activity; negative regulation of translation; neuron apoptosis; positive regulation by organism of apoptotic process in other organism involved in symbiotic interaction; positive regulation of cytokine secretion; protein stabilization; regulation of macroautophagy
Research Articles on GAPDH
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Product Notes
The Human GAPDH gapdh (Catalog #AAA285422) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA285422 ELISA Kit recognizes Human GAPDH. It is sometimes possible for the material contained within the vial of "Glyceraldehyde-3-phosphate dehydrogenase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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