GALNT7 recombinant protein

GALNT7 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121827

• Gene Names: GALNT7; GalNAcT7; GALNAC-T7
• Purity: >85% as determined by SDS-PAGE
• Synonyms: GALNT7; GALNT7 Protein; Human; Recombinant (His Tag); Human GALNT7 Protein (His Tag); GALNAC-T7 Protein; GalNAcT7 Protein; polypeptide N-acetylgalactosaminyltransferase 7; GALNT7 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 150mM NaCl, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Pro30-Val657

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Pro 30
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human GALNT7 (NP_059119.2)(Pro30-Val657) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for GALNT7 recombinant protein

Background: GalNAc-transferase-7 (GALNT7) is essential for the regulation of cell proliferation and has been implicated in tumorigenesis. Colorectal cancer (CRC) arises in a multistep molecular network process, which is from either discrete genetic perturbation or epigenetic dysregulation. GALNT7 acts as a glycosyltransferase in protein O-glycosylation, involving in the occurrence and development of CRC. GALNT7 silencing significantly attenuated the proliferation, clonogenicity and migration of LSCC cells and induced their cycling arrest. miR-30e may function as tumor suppressors in cervical cancer through downregulation of GALNT7. Both miR-30e and its novel target, GALNT7, may play an important role in the process of cervical cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 157502212
• NCBI GeneID: 51809
• NCBI Accession #: NP_059119.2
• NCBI GenBank Nucleotide #: NM_017423.2
• UniProt Accession #: Q86SF2; Q7Z5W7; Q9UJ28; B3KQU3
• Molecular Weight: 75,389 Da
• NCBI Official Full Name: N-acetylgalactosaminyltransferase 7
• NCBI Official Synonym Full Names: polypeptide N-acetylgalactosaminyltransferase 7
• NCBI Official Symbol: GALNT7
• NCBI Official Synonym Symbols: GalNAcT7; GALNAC-T7
• NCBI Protein Information: N-acetylgalactosaminyltransferase 7; pp-GaNTase 7; polypeptide GalNAc transferase 7; protein-UDP acetylgalactosaminyltransferase 7; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 7; UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactos
• UniProt Protein Name: N-acetylgalactosaminyltransferase 7
• Protein Family: N-acetylgalactosaminyltransferase
• UniProt Gene Name: GALNT7
• UniProt Synonym Gene Names: GalNAc-T7; pp-GaNTase 7
• UniProt Entry Name: GALT7_HUMAN

NCBI Description

This gene encodes GalNAc transferase 7, a member of the GalNAc-transferase family. The enzyme encoded by this gene controls the initiation step of mucin-type O-linked protein glycosylation and transfer of N-acetylgalactosamine to serine and threonine amino acid residues. This enzyme is a type II transmembrane protein and shares common sequence motifs with other family members. Unlike other family members, this enzyme shows exclusive specificity for partially GalNAc-glycosylated acceptor substrates and shows no activity with non-glycosylated peptides. This protein may function as a follow-up enzyme in the initiation step of O-glycosylation. [provided by RefSeq, Jul 2008]

Uniprot Description

GALNT7: a glycopeptide transferase involved in O-linked oligosaccharide biosynthesis, which catalyzes the transfer of an N-acetyl-D-galactosamine residue to an already glycosylated peptide. A single-pass type II membrane protein that localizes to the Golgi apparatus membrane. In contrast to other proteins of the family, it does not act as a peptide transferase that transfers GalNAc onto serine or threonine residue on the protein receptor, but instead requires the prior addition of a GalNAc on a peptide before adding additional GalNAc moieties.

Protein type: EC 2.4.1.-; Glycan Metabolism - O-glycan biosynthesis; Transferase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q31.1

Cellular Component: Golgi membrane; membrane; integral to membrane

Molecular Function: metal ion binding; polypeptide N-acetylgalactosaminyltransferase activity; carbohydrate binding

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; carbohydrate metabolic process; post-translational protein modification