Typical Testing Data/Standard Curve (for reference only)
Human Anti Glutamic Acid Decarboxylase 67 antibody ELISA Kit | GAD67 elisa kit
Human Anti Glutamic Acid Decarboxylase 67 antibody ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: GAD65Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing Glutamic Acid Decarboxylase 65 antigen and an GAD65Ab-HRP conjugate. The assay sample and buffer are incubated together with GAD65Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GAD65Ab concentration since GAD65Ab from samples and GAD65Ab-HRP conjugate compete for the Glutamic Acid Decarboxylase 65 antigen binding site. Since the number of sites is limited, as more sites are occupied by GAD65Ab from the sample, fewer sites are left to bind GAD65Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GAD65Ab concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008]
Uniprot Description
GAD67: Catalyzes the production of GABA. Homodimer. Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. Belongs to the group II decarboxylase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Mitochondrial; EC 4.1.1.15; Other Amino Acids Metabolism - beta-alanine; Lyase; Carbohydrate Metabolism - butanoate; Other Amino Acids Metabolism - taurine and hypotaurine
Chromosomal Location of Human Ortholog: 2q31
Cellular Component: plasma membrane; intracellular; vesicle membrane
Molecular Function: protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; protein N-terminus binding; pyridoxal phosphate binding
Biological Process: response to drug; synaptic transmission; glutamate catabolic process; glutamate decarboxylation to succinate; neurotransmitter secretion; gamma-aminobutyric acid biosynthetic process; protein-pyridoxal-5-phosphate linkage; neurotransmitter biosynthetic process
Disease: Cerebral Palsy, Spastic Quadriplegic, 1
Research Articles on GAD67
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Product Notes
The Human GAD67 gad1 (Catalog #AAA720934) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA720934 ELISA Kit recognizes Human GAD67. It is sometimes possible for the material contained within the vial of "Anti Glutamic Acid Decarboxylase 67 antibody, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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