Frizzled-8 (fzd8) Recombinant Protein | fzd8-a recombinant protein

Recombinant Xenopus laevis Frizzled-8 (fzd8)

• Gene Names: fzd8-a; Xfz8; fzd8; frizzled8
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Frizzled-8 (fzd8); Recombinant Xenopus laevis Frizzled-8 (fzd8); Recombinant Frizzled-8 (fzd8); Frizzled-8; Fz-8; Xfz8; fzd8-a recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 24-581
• Sequence: KELSCQEITVPLCKDIGYNYTYMPNQFNHDTQDEAGMEVHQFWPLVVIHCSPDLKFFLCSMYTPICLEDYKKPLPPCRSVCERARAGCAPLMRQYGFAWPDRMRCDRLPEQGNPDTLCMDYYNRTEQTTAAPSHPEPPKPPARSVPKGRTRVEPPRSRSRATGCESGCQCRAPMVQVSNERHPLYNRVRTGQIPNCAMPCHNPFFSPEERTFTEFWIGLWSVLCFASTFATVSTFLIDMERFKYPERPIIFLSACYLLVSTGYLIRLIAGHEKVACSRGELDLEHIIHYETTGPALCTLVFLLIYFFGMASSIWWVILSLTWFLAAGMKWGNEAIAGYSQYFHLAAWLVPSIKSIAVLALSSVDGDPVAGICFVGNQNLDNLRGFVLAPLVIYLFIGSMFLLAGFVSLFRIRSVIKQGGTKTDKLEKLMIRIGIFSVLYTVPATIVVACFFYEQHNRQGWEVAHNCNSCQPEMAQPHRPDYAVFMLKYFMCLVVGITSGVWIWSGKTLESWRAFCTRCCWGSKATGGSMYSDVSTGLTWRSGTGSSVSCPKQMPLSQV
• Sequence Length: 581

• Species: Xenopus laevis (African clawed frog)
• Preparation and Storage: Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

NCBI and Uniprot Product Information

• NCBI GI #: 147900366
• NCBI GeneID: 373690
• NCBI Accession #: NP_001079144.1
• NCBI GenBank Nucleotide #: NM_001085675.1
• UniProt Accession #: O93274; Q9YI55
• Molecular Weight: 65,379 Da
• NCBI Official Full Name: frizzled-8
• NCBI Official Synonym Full Names: frizzled family receptor 8
• NCBI Official Symbol: fzd8-a
• NCBI Official Synonym Symbols: Xfz8; fzd8; frizzled8
• NCBI Protein Information: frizzled-8; fz-8; frizzled 8 protein; frizzled homolog 8
• UniProt Protein Name: Frizzled-8
• UniProt Gene Name: fzd8
• UniProt Synonym Gene Names: fz8; Fz-8; Xfz8
• UniProt Entry Name: FZD8_XENLA

Uniprot Description

Function: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activation by Wnt8, Wnt5A or Wnt3A induces expression of beta-catenin target genes. Displays an axis-inducing activity. Ref.3

Subcellular location: Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein

By similarity Ref.3.

Developmental stage: First expressed at high levels in the late blastula stages. At early gastrula, expressed in the deep cells of the Spemann organizer prior to involution of the dorsal blastopore lip. Detected in presumptive neurectoderm as gastrulation proceeds. Becomes restricted to the anterior ectoderm by the end of gastrulation. At neurula stages, localized in the most anterior region of the embryo, mainly in the anterior ectoderm including telencephalic and cement gland regions.

Domain: Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway

By similarity.The FZ domain is involved in binding with Wnt ligands

By similarity.

Sequence similarities: Belongs to the G-protein coupled receptor Fz/Smo family.Contains 1 FZ (frizzled) domain.