FSCN1 recombinant protein

FSCN1, 1-493aa, Human, His tag, E Coli

Cat. Num. AAA203522

• Gene Names: FSCN1; HSN; SNL; p55; FAN1
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: FSCN1; 1-493aa; Human; His tag; E Coli; Fascin; FAN1; HSN; SNL; FSCN1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 100mM NaCl
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MTANGTAEAV QIQFGLINCG NKYLTAEAFG FKVNASASSL KKKQIWTLEQ PPDEAGSAAV CLRSHLGRYL AADKDGNVTC EREVPGPDCR FLIVAHDDGR WSLQSEAHRR YFGGTEDRLS CFAQTVSPAE KWSVHIAMHP QVNIYSVTRK RYAHLSARPA DEIAVDRDVP WGVDSLITLA FQDQRYSVQT ADHRFLRHDG RLVARPEPAT GYTLEFRSGK VAFRDCEGRY LAPSGPSGTL KAGKATKVGK DELFALEQSC AQVVLQAANE RNVSTRQGMD LSANQDEETD QETFQLEIDR DTKKCAFRTH TGKYWTLTAT GGVQSTASSK NASCYFDIEW RDRRITLRAS NGKFVTSKKN GQLAASVETA GDSELFLMKL INRPIIVFRG EHGFIGCRKV TGTLDANRSS YDVFQLEFND GAYNIKDSTG KYWTVGSDSA VTSSGDTPVD FFFEFCDYNK VAIKVGGRYL KGDHAGVLKA SAETVDPASL WEY
• Sequence Length: 1-493

• Applicable Applications for FSCN1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for FSCN1 recombinant protein

FSCN1 is an actin-bundling protein that provides rigidity to filopodial bundles to efficiently push the membrane forward during cytoskeleton remodeling and cell migration. Probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers. And this protein is absent from most normal epithelia yet is upregulated in multiple forms of human carcinoma, where its expression correlates clinically with a poor prognosis. FSCN1 expression has been localized to neurons, glial cells, and endothelial cells. Recombinant human FSCN1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for FSCN1 recombinant protein

Cytoskeleton

References

Directional transport by nonprocessive motor proteins on fascin-cross-linked actin arrays. Yongkuk Lee & Parviz Famouri (2013)

NCBI and Uniprot Product Information

• NCBI GI #: 4507115
• NCBI GeneID: 6624
• NCBI Accession #: NP_003079
• NCBI GenBank Nucleotide #: NM_003088.3
• UniProt Accession #: Q16658; Q96IC5; Q96IH1; Q9BRF1; A6NI89; B2RE97
• Molecular Weight: 56.7kDa (513aa)
• NCBI Official Full Name: fascin
• NCBI Official Synonym Full Names: fascin actin-bundling protein 1
• NCBI Official Symbol: FSCN1
• NCBI Official Synonym Symbols: HSN; SNL; p55; FAN1
• NCBI Protein Information: fascin
• UniProt Protein Name: Fascin
• Protein Family: Fascin
• UniProt Gene Name: FSCN1
• UniProt Synonym Gene Names: FAN1; HSN; SNL

NCBI Description

This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15. [provided by RefSeq, Sep 2011]

Uniprot Description

Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration.