Canine Fibroblast Growth Factor Receptor SubstRate 2 ELISA Kit | FRS2 elisa kit

Canine Fibroblast Growth Factor Receptor SubstRate 2 ELISA Kit

Cat. Num. AAA736155

• Gene Names: FRS2; SNT; SNT1; FRS2A; SNT-1; FRS2alpha
• Reactivity: Canine
• Synonyms: Fibroblast Growth Factor Receptor SubstRate 2; Canine Fibroblast Growth Factor Receptor SubstRate 2 ELISA Kit; FRS2 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 25-500pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This FRS2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine FRS2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FRS2 elisa kit

Principle of the Assay: FRS2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FRS2 antibody and an FRS2-HRP conjugate. The assay sample and buffer are incubated together with FRS2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FRS2 concentration since FRS2 from samples and FRS2-HRP conjugate compete for the anti-FRS2 antibody binding site. Since the number of sites is limited, as more sites are occupied by FRS2 from the sample, fewer sites are left to bind FRS2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FRS2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for FRS2 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 110347408
• NCBI GeneID: 10818
• NCBI Accession #: NP_006645.3
• NCBI GenBank Nucleotide #: NM_006654.4
• Molecular Weight: 57,029 Da
• NCBI Official Full Name: fibroblast growth factor receptor substrate 2
• NCBI Official Synonym Full Names: fibroblast growth factor receptor substrate 2
• NCBI Official Symbol: FRS2
• NCBI Official Synonym Symbols: SNT; SNT1; FRS2A; SNT-1; FRS2alpha
• NCBI Protein Information: fibroblast growth factor receptor substrate 2; FGFR substrate 2; FGFR signalling adaptor; FGFR-signaling adaptor SNT; suc1-associated neurotrophic factor target 1
• UniProt Protein Name: Fibroblast growth factor receptor substrate 2
• Protein Family: Fibroblast growth factor receptor substrate
• UniProt Gene Name: FRS2
• UniProt Synonym Gene Names: FGFR substrate 2; SNT-1
• UniProt Entry Name: FRS2_HUMAN

Uniprot Description

FRS2: an adaptor protein involved in fibroblast growth factor receptor (FGFR) signaling. Plays an important role in linking FGFR and nerve growth factor receptors with Ras/MAPK signaling pathways.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 12q15

Cellular Component: membrane; integral to plasma membrane; plasma membrane; intercellular junction; endosome

Molecular Function: protein binding; phosphatase activator activity; neurotrophin TRKA receptor binding; transmembrane receptor protein tyrosine kinase adaptor protein activity; insulin receptor binding; fibroblast growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; activation of MAPKK activity; activation of MAPK activity; gastrulation with mouth forming second; regulation of epithelial cell proliferation; regulation of apoptosis; G-protein coupled receptor protein signaling pathway; determination of anterior/posterior axis, embryo; induction of an organ; forebrain development; neuroblast proliferation; insulin receptor signaling pathway; innate immune response; optic placode formation involved in camera-type eye; transmembrane receptor protein tyrosine phosphatase signaling pathway