Mouse Forkhead box protein P3 ELISA Kit | Foxp3 elisa kit

Mouse Forkhead box protein P3 ELISA Kit

Cat. Num. AAA2885344

• Gene Names: Foxp3; sf; JM2; scurfin
• Reactivity: Mouse
• Synonyms: Forkhead box protein P3; Mouse Forkhead box protein P3 ELISA Kit; Scurfin; Foxp3; Foxp3 elisa kit

• Reactivity: Mouse
• Sequence Length: 429

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Foxp3 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 313569800
• NCBI GeneID: 20371
• NCBI Accession #: NP_001186276.1
• NCBI GenBank Nucleotide #: NM_001199347.1
• UniProt Accession #: Q99JB6
• Molecular Weight: 47,346 Da
• NCBI Official Full Name: forkhead box protein P3
• NCBI Official Synonym Full Names: forkhead box P3
• NCBI Official Symbol: Foxp3
• NCBI Official Synonym Symbols: sf; JM2; scurfin
• NCBI Protein Information: forkhead box protein P3
• UniProt Protein Name: Forkhead box protein P3
• Protein Family: Forkhead box protein
• UniProt Gene Name: Foxp3
• UniProt Entry Name: FOXP3_MOUSE

NCBI Description

The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene result in the scurfy phenotype (sf). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

FOXP3: Probable transcription factor. Plays a critical role in the control of immune response. Interacts with IKZF3. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor; C2H2-type zinc finger protein; Cell cycle regulation

Cellular Component: cytoplasm; intracellular; nucleus

Molecular Function: DNA binding; histone acetyltransferase binding; histone deacetylase binding; metal ion binding; NFAT protein binding; nucleic acid binding; protein binding; protein homodimerization activity; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription corepressor activity; transcription factor activity

Biological Process: anatomical structure morphogenesis; B cell homeostasis; CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation; CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment; cytokine production; inhibition of CREB transcription factor; inhibition of NF-kappaB transcription factor; myeloid cell homeostasis; negative regulation of cell proliferation; negative regulation of chronic inflammatory response; negative regulation of cytokine biosynthetic process; negative regulation of cytokine secretion; negative regulation of histone acetylation; negative regulation of histone deacetylation; negative regulation of immune response; negative regulation of inflammatory response; negative regulation of interferon-gamma biosynthetic process; negative regulation of interferon-gamma production; negative regulation of interleukin-10 production; negative regulation of interleukin-17 production; negative regulation of interleukin-2 biosynthetic process; negative regulation of interleukin-2 production; negative regulation of interleukin-4 production; negative regulation of interleukin-5 production; negative regulation of interleukin-6 production; negative regulation of isotype switching to IgE isotypes; negative regulation of lymphocyte proliferation; negative regulation of T cell cytokine production; negative regulation of T cell proliferation; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of tumor necrosis factor production; positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation; positive regulation of histone acetylation; positive regulation of immature T cell proliferation in the thymus; positive regulation of interleukin-4 production; positive regulation of peripheral T cell tolerance induction; positive regulation of regulatory T cell differentiation; positive regulation of T cell anergy; positive regulation of T cell tolerance induction; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of transforming growth factor-beta1 production; regulation of immunoglobulin production; regulation of isotype switching to IgG isotypes; regulation of T cell anergy; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; regulatory T cell differentiation; T cell activation; T cell mediated immunity; T cell receptor signaling pathway; tolerance induction; tolerance induction to self antigen; transcription, DNA-dependent