Rat FOS Like Antigen 1 ELISA Kit | FOSL1 elisa kit

Rat FOS Like Antigen 1 ELISA Kit

Cat. Num. AAA729346

• Reactivity: Rat
• Synonyms: FOS Like Antigen 1; Rat FOS Like Antigen 1 ELISA Kit; FOSL1 elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This FOSL1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat FOSL1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FOSL1 elisa kit

Principle of the assay: FOSL1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FOSL1 antibody and an FOSL1-HRP conjugate. The assay sample and buffer are incubated together with FOSL1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FOSL1 concentration since FOSL1 from samples and FOSL1-HRP conjugate compete for the anti-FOSL1 antibody binding site. Since the number of sites is limited, as more sites are occupied by FOSL1 from the sample, fewer sites are left to bind FOSL1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FOSL1 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4885243
• NCBI GeneID: 8061
• NCBI Accession #: NP_005429.1
• NCBI GenBank Nucleotide #: NM_005438.4
• NCBI Official Full Name: fos-related antigen 1 isoform 1
• Protein Family: Fos-related antigen

NCBI Description

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

Uniprot Description

FRA1: an oncogenic transcription factor of the bZIP family, Fos subfamily. The expression of Fos proteins is rapidly and transiently induced by a variety of extracellular stimuli including growth factors, cytokines, neurotransmitters, polypeptide hormones, and stress. Fos proteins dimerize with Jun proteins (c-Jun, JunB, and JunD) to form Activator Protein-1 (AP-1), a transcription factor that binds to TRE/AP-1 elements and activates transcription. Fos and Jun proteins contain the leucine-zipper motif that mediates dimerization and an adjacent basic domain that binds to DNA. The various Fos/Jun heterodimers differ in their ability to transactivate AP-1 dependent genes. In addition to increased expression, phosphorylation of Fos proteins by Erk kinases in response to extracellular stimuli may further increase transcriptional activity. Following growth factor stimulation, expression of FosB and c-Fos in quiescent fibroblasts is immediate but short-lived, while FRA1 and FRA2 expression persists longer. FRA1 is involved in cell motility, invasiveness, and inhibits apoptosis. Elevated in many cancers and associated with tumorigenesis and cancer progression. Involved in Erk2-mediated Epithelial-to-Mesenchymal Transition (EMT) pathway. ERK2/FRA2 regulate ZEB1/2 expression, known to be associated with the EMT. Smad2/3-Fra1 complexes may reflect activation of the Smad/AP-1-dependent TGF¿-induced breast cancer invasion program. Activation of FRA1/C-JUN by ERK/AKT pathways can induce EZH2 overexpression, silencing integrin alpha-2 expression, and increasing the metastatic potential of colorectal cancer. Belongs to the bZIP family. Fos subfamily.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: presynaptic membrane; nucleoplasm; neuron projection; cytosol; nucleus

Molecular Function: protein binding; transcription factor activity

Biological Process: response to drug; transcription from RNA polymerase II promoter; response to gravity; response to cAMP; in utero embryonic development; positive regulation of apoptosis; response to virus; vitellogenesis; positive regulation of cell cycle; learning; female pregnancy; chemotaxis; regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; response to hydrogen peroxide; response to mechanical stimulus; response to cytokine stimulus; positive regulation of cell proliferation; cellular response to extracellular stimulus; cellular defense response; positive regulation of transcription factor activity; response to progesterone stimulus; response to corticosterone stimulus