FLT3 recombinant protein

FLT3 Protein, Human, Recombinant (ECD, hFc Tag)

Cat. Num. AAA8120270

• Gene Names: FLT3; FLK2; STK1; CD135; FLK-2
• Purity: >95% as determined by SDS-PAGE
• Synonyms: FLT3; FLT3 Protein; Human; Recombinant (ECD; hFc Tag); Human FLT3 Protein (ECD; Fc Tag); CD135 Protein; FLK-2 Protein; FLK2 Protein; STK1 Protein; fms-related tyrosine kinase 3; FLT3 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Asn27-Ser543

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Asn 27
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human FLT3 (NP_004110.2)(Asn27-Ser543) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for FLT3 recombinant protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD135, also known as FLT-3, FLK-2, is a member of the CD system. CD135 is an important cell surface marker recognized by specific sets of antibodies to identify the types of hematopoietic (blood) progenitors in the bone marrow and it function to differentiate hematopoietic stem cells, which are CD135 negative, from multipotent progenitors, which are CD135 positive. CD135 is a receptor tyrosine kinase type I¢ for the cytokine Flt3 ligand and activat signaling through second messengers by binding to Flt3. Signaling through CD135 is important for lymphocyte development. The encoding gene CD135 is a proto-oncogene to which mutations happened will lead to cancer such as leukemia.

Product Categories/Family for FLT3 recombinant protein

Cancer Drug Targets Receptor Tyrosine Kinases (RTKs)

References

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
Bertho, et al. (2000) CD135 (Flk2/Flt3) Expression by Human Thymocytes Delineates a Possible Role of FLT3-Ligand in T-Cell Precursor Proliferation and Differentiation. Scandinavian Journal of Immunology. 52 (1): 53-61.

NCBI and Uniprot Product Information

• NCBI GI #: 121114304
• NCBI GeneID: 2322
• NCBI Accession #: NP_004110.2
• NCBI GenBank Nucleotide #: NM_004119.2
• UniProt Accession #: P36888; Q13414; A0AVG9; B7ZLT7; B7ZLT8; F5H0A0
• Molecular Weight: 112,903 Da
• NCBI Official Full Name: receptor-type tyrosine-protein kinase FLT3
• NCBI Official Synonym Full Names: fms-related tyrosine kinase 3
• NCBI Official Symbol: FLT3
• NCBI Official Synonym Symbols: FLK2; STK1; CD135; FLK-2
• NCBI Protein Information: receptor-type tyrosine-protein kinase FLT3; STK-1; CD135 antigen; FL cytokine receptor; fetal liver kinase 2; fms-like tyrosine kinase 3; stem cell tyrosine kinase 1; growth factor receptor tyrosine kinase type III
• UniProt Protein Name: Receptor-type tyrosine-protein kinase FLT3
• Protein Family: Flt3 receptor-interacting lectin
• UniProt Gene Name: FLT3
• UniProt Synonym Gene Names: CD135; FLK2; STK1; FLK-2; FLT-3; STK-1
• UniProt Entry Name: FLT3_HUMAN

NCBI Description

This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia. [provided by RefSeq, Jul 2008]

Uniprot Description

FLT3: a receptor tyrosine kinase of the PDGFR family that binds the FL cytokine. FLT3 -/- mice have an impaired developmental capacity of primitive hematopoietic progenitor cells of all lineages with the greatest impact on lymphopoietic precursors. Activating mutations found in one third of cases of acute myeloid leukemia (AML), as well as in acute lymphoblastic leukemia, acute promyelocytic leukemia and myelodysplastic syndrome. Inhibitors: Sutent and PKC412.

Protein type: Kinase, protein; Membrane protein, integral; EC 2.7.10.1; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; PDGFR family

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: protein complex; integral to plasma membrane; endoplasmic reticulum lumen; cytosol; nucleus; external side of plasma membrane

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; vascular endothelial growth factor receptor activity; protein binding; protein homodimerization activity; protein complex binding; phosphoinositide 3-kinase binding; transmembrane receptor protein tyrosine kinase activity; ATP binding; glucocorticoid receptor binding

Biological Process: negative regulation of B cell differentiation; lymphocyte proliferation; pro-B cell differentiation; organ regeneration; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; cytokine and chemokine mediated signaling pathway; positive regulation of phosphoinositide 3-kinase activity; response to organic nitrogen; positive regulation of phosphoinositide 3-kinase cascade; regulation of apoptosis; negative regulation of cell proliferation; positive regulation of MAP kinase activity; positive regulation of tyrosine phosphorylation of STAT protein; myeloid progenitor cell differentiation; positive regulation of MAPKKK cascade; B cell differentiation; positive regulation of cell proliferation; hemopoiesis; pro-T cell differentiation; transmembrane receptor protein tyrosine kinase signaling pathway; leukocyte homeostasis

Disease: Leukemia, Acute Lymphoblastic