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SDS-Page (Figure. SDS-PAGE)

Flotillin 2 Recombinant Protein | FLOT2 recombinant protein

Recombinant Flotillin 2 (FLOT2)

Gene Names
FLOT2; ESA; ECS1; ESA1; ECS-1; M17S1
Applications
SDS-Page, Western Blot
Purity
>90%
Synonyms
Flotillin 2; Recombinant Flotillin 2 (FLOT2); ECS1; ESA; ESA1; M17S1; Epidermal Surface Antigen 1; Membrane Component; Chromosome 17; Surface Marker 1; 35kD Protein Identified By Monoclonal Antibody ECS-1; FLOT2 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
>90%
Form/Format
Freeze-dried powder
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Sequence Positions
Ala35~Asp379
Sequence Length
428
Applicable Applications for FLOT2 recombinant protein
Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
Source
Prokaryotic expression
Species
Human
Tag
N-terminal His Tag
Subcellular Location
Membrane
Isoelectric Point
4.8
Reconstitution
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

(Figure. SDS-PAGE)

SDS-Page (Figure. SDS-PAGE)
Product Categories/Family for FLOT2 recombinant protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 42.1kDa
Accurate Molecular Mass: 44kDa
NCBI Official Full Name
Flotillin-2
NCBI Official Synonym Full Names
flotillin 2
NCBI Official Symbol
FLOT2
NCBI Official Synonym Symbols
ESA; ECS1; ESA1; ECS-1; M17S1
NCBI Protein Information
flotillin-2
UniProt Protein Name
Flotillin-2
Protein Family
UniProt Gene Name
FLOT2
UniProt Synonym Gene Names
ESA1; M17S1; ESA
UniProt Entry Name
FLOT2_HUMAN

NCBI Description

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. This gene encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling. [provided by RefSeq, Jul 2008]

Uniprot Description

FLOT2: May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function. Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2. Interacts with ECM29. In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes. Belongs to the band 7/mec-2 family. Flotillin subfamily.

Protein type: Cell adhesion

Chromosomal Location of Human Ortholog: 17q11-q12

Cellular Component: focal adhesion; membrane; endocytic vesicle; perinuclear region of cytoplasm; plasma membrane; caveola; flotillin complex; vesicle; endosome; acrosomal membrane

Molecular Function: ionotropic glutamate receptor binding; protein binding; protease binding

Biological Process: epidermis development; protein stabilization; cell adhesion

Research Articles on FLOT2

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Product Notes

The FLOT2 flot2 (Catalog #AAA2122962) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Ala35~Asp379. AAA Biotech's Flotillin 2 can be used in a range of immunoassay formats including, but not limited to, Positive Control, Immunogen, SDS-PAGE, Western Blot (WB). Researchers should empirically determine the suitability of the FLOT2 flot2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Flotillin 2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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