Human Fibrinogen-like protein 1 ELISA Kit | FGL1 elisa kit
Human Fibrinogen-like protein 1 (FGL1) ELISA Kit
Gene Names
FGL1; HFREP1; HP-041; LFIRE1; LFIRE-1
Reactivity
Human
Synonyms
Fibrinogen-like protein 1; Human Fibrinogen-like protein 1 (FGL1) ELISA Kit; FGL1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human FGL1. No significant cross-reactivity or interference between Human FGL1 and analogues was observed.
Sequence Length
312
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
78.1 pg/mL - 5000 pg/mL
Sensitivity
26 pg/mL (mean of 6 independent assays)
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for FGL1 elisa kit
Intended Uses: For the quantitative detection of Human Fibrinogen-like protein 1 (FGL1) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate FGL1 in Human serum, plasma. An antibody specific for FGL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FGL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate FGL1 in Human serum, plasma. An antibody specific for FGL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FGL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,379 Da
NCBI Official Full Name
fibrinogen-like protein 1
NCBI Official Synonym Full Names
fibrinogen like 1
NCBI Official Symbol
FGL1
NCBI Official Synonym Symbols
HFREP1; HP-041; LFIRE1; LFIRE-1
NCBI Protein Information
fibrinogen-like protein 1
UniProt Protein Name
Fibrinogen-like protein 1
Protein Family
UniProt Gene Name
FGL1
UniProt Synonym Gene Names
HFREP1; HFREP-1; LFIRE-1
NCBI Description
Fibrinogen-like 1 is a member of the fibrinogen family. This protein is homologous to the carboxy terminus of the fibrinogen beta- and gamma- subunits which contains the four conserved cysteines of fibrinogens and fibrinogen related proteins. However, this protein lacks the platelet-binding site, cross-linking region and a thrombin-sensitive site which are necessary for fibrin clot formation. This protein may play a role in the development of hepatocellular carcinomas. Four alternatively spliced transcript variants encoding the same protein exist for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
FGL1: Has hepatocyte mitogenic activity.
Protein type: Extracellular matrix; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 8p22
Cellular Component: fibrinogen complex
Research Articles on FGL1
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Product Notes
The Human FGL1 fgl1 (Catalog #AAA280295) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA280295 ELISA Kit recognizes Human FGL1. It is sometimes possible for the material contained within the vial of "Fibrinogen-like protein 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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